I am having a horrible problem culturing Nano. I am good for about 10 days then it turns brown and dies. Does the type of salt mix you use help or hinder algae growth. Right now I am using reef crystals and was wondering if it contains something the nano doesn't like.
Here is my procedure:
The well water Water is sterilzed. I have boiled it, I have used bleach then dechlorinator (sodium Thiosulfate), RO water before the Di resin and after the resin. (used one of each of these methods for at least two trials each )
All containers and air hoses are sterilized with bleach water and with alcohol
Florida aqua farms culture disk soaked in sterile water for 24 hours
added to 2 liters of boiled water(that's cooled down) with a salinity of 1.020
3ml of f/2 to 2 liters of water
filtered air (carbon and sterile cotton)
light from 2 brand new 2 bulb 4 ft fluorescent 6500 k bulbs (I have 8 one gallon glass jugs)(24hours)(The fixtures are old but bulbs are new)
After 7-8 days I add enough sterile water to make a gallon then continue with light and air
3 ml of f/2
(I also tried using 1 ml of f/2 in beginning and 1 ml at split with same results)
Day 10 dead.
This is getting expensive and time consuming for growing rotifers to feed to baby clownfish.
Questions:
The area that I am culturing is in the basement with a constant temp of about 75. Could the air have mold or bacteria in it that causes a problem?
Could older ballast not provide enough power to benefit from the 6500 k bulbs?
Is the salinity to high? (Trying to stay close to the feeding tank conditions)
Could my F/2 be contaminated?
Should I beat my head harder on the wall?
Any feedback would be greatly appreciated except the beating of my head part.
Scott
Here is my procedure:
The well water Water is sterilzed. I have boiled it, I have used bleach then dechlorinator (sodium Thiosulfate), RO water before the Di resin and after the resin. (used one of each of these methods for at least two trials each )
All containers and air hoses are sterilized with bleach water and with alcohol
Florida aqua farms culture disk soaked in sterile water for 24 hours
added to 2 liters of boiled water(that's cooled down) with a salinity of 1.020
3ml of f/2 to 2 liters of water
filtered air (carbon and sterile cotton)
light from 2 brand new 2 bulb 4 ft fluorescent 6500 k bulbs (I have 8 one gallon glass jugs)(24hours)(The fixtures are old but bulbs are new)
After 7-8 days I add enough sterile water to make a gallon then continue with light and air
3 ml of f/2
(I also tried using 1 ml of f/2 in beginning and 1 ml at split with same results)
Day 10 dead.
This is getting expensive and time consuming for growing rotifers to feed to baby clownfish.
Questions:
The area that I am culturing is in the basement with a constant temp of about 75. Could the air have mold or bacteria in it that causes a problem?
Could older ballast not provide enough power to benefit from the 6500 k bulbs?
Is the salinity to high? (Trying to stay close to the feeding tank conditions)
Could my F/2 be contaminated?
Should I beat my head harder on the wall?
Any feedback would be greatly appreciated except the beating of my head part.
Scott