1% Ivermectin (in-tank) Treatment for Coral Boring Spionid Worms

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Disclaimer: Do not try this at home. It’s not reef safe. I will not take any responsibility for anyone crashing their system. I DO NOT recommend anybody try this. :)


Ivermectin used: AGRI-MECTIN 1%
Potency: (1 mL = 10mg)
Expiration date: 11/24

Start Date: Sunday 07/28/24

System info: 105/G Rimless 48x24x21 Planet Aquarium. I dose it as 100/G even including Sump volume.

System Age: 2.6 years.

Rock: Marco Dry Rock. 10lbs of KP Aquatics Starter Rock was added later.

Current Fish: (2) Tomini Tangs, (1) Blue Regal Tang, (1) Azure Damsel, (2) Yellow Tail Damsels, (1) Bicolor Chromis, & (1) Diadema Dottyback.

Test Kits Used:
Hanna Alkalinity, Hanna Phosphorus ULR, Hanna High Range Nitrate, Salifert Calcium, Salifert Magnesium.

ICP: OCEAMO ICP-MS.

Pre-Treatment Values:
Sunday 07/28 3:30pm:
KH = 8.5, P = 0.138, N = 15.8.
7/17 Ca = 440 (ICP-MS result)
7/17 Mg = 1336 (ICP-MS result)

Also have Pre-Treatment ICP-MS (07/17) and Aquabiomics Microbiome Results (Pre/Post) for those interested when they result.

Sunday 8:00pm Pre-check: GAC loaded into fluidized reactor. Fines flushed out. (2) Power-filters deployed on LT and RT side of DT near front glass. 60 gallons of water change water made and ready. Hospital tank setup and ready. Skimmer raised up 6” on (2) different skimmer stands. Valve fully opened and skimmer dialed down. Skimmer cup removed. Siphon hoses positioned close to tank. 8” Filter cup loaded in drain chamber and ready with Sera Filter wool. Extra wool pre-cut and ready. (3) fine mesh fish nets ready near tank.

8:07pm. Dosed 1mL AGRI-MECTIN (1% Ivermectin) into return chamber.

8:17pm. No changes or death noted in first 10 minutes. Skimmer operation is normal. No bubbles in sump.

8:22pm. Dosed another 2mL into return chamber.

8:29pm. Still no changes. Limpet snails are calm. Nothing crawling around. I see some Spionid worms tentacles out like everything is normal. No dead pods or brittle stars yet. I can see my large brittle star under the rocks, and his legs haven’t moved. He appears to be unaffected so far.

8:33pm. Two dead Amphipods noted flying by front glass. Power-filters and Air-stones turned on.

8:35pm. More dead Amphipods and other tiny pods populating in water column and flying around. A couple baby bristle worms also dead floating around. So far only 3mL dosed.

8:37pm. Dosed another 2mL. Skimmer operation ok. No overflowing or excessive bubbles. Fish eating dead pods and worms. Limpet snails still unaffected.

8:50pm. The Limpet snail I’ve been watching by front glass just blew away in the flow. Others are now crawling around. Acropora Florida, WWC Heartbreaker, TGC Cherry Bomb losing PE. Other Tenuis are fine. No slime or cloudy water at all.

8:53pm. Dosed another 2mL.

8:57pm. A few Limpets flying around water column with pods and worms.

8:59pm. Bill Murray loosing PE with mild reduction on other Acro’s. Goni retracted. Rainbow BTA retracted tentacles. Fish doing ok so far.

9:14pm. Dosed another 3mL. 10mL total up until now.

9:46pm. Just finished siphoning the entire tank. A lot of dead Limpet snails on the rocks also. Not one snail left on back glass. Every one of them fell to bottom of tank. Tons of dead worms and various pods now. Skimmer still not overflowing, but bubbles are at top of neck. No bubbles in the sump. I noticed the water did cloud briefly after the last 3mL dose, but is clear now. Looking at Hydros graph, there’s no significant change in pH.

10:02pm. Minimal stuff flying around after siphoning. Water is clear. PE coming back on some Acro’s. Goni is still retracted. Never saw excessive slime from Acro’s. Maybe keeping the skimmer on continuously with air-stones was key, but also spreading out the doses instead of one large dose. I see slime coming from Spionid worm tubes. I don’t see any worms coming out or hanging out at this point. I will likely dose another 2mL here shortly. I want to be sure I get them.

10:15pm. I continue to see more baby bristle worms and Limpets floating around. They must die in stages. There’s other small worms hanging out of the rocks dead. A lot of worms will die inside of the rocks or get pushed under the rocks which is why siphoning is important. Fish are swimming around like normal so far. Currently I’m at 10mL to 100/G volume. This will probably get the Spionid worms, but I’m not sure how long it will take, and I don’t want to leave the Ivermectin in for more than 12-14 hrs or so. So I will probably dose up at little higher. Corals seem to tolerate just fine with minimal stress. Rainbow BTA put tentacles back out.

11:15pm. Dosed another 2mL. Total is 12mL now. Continuing to siphon a few worms and Limpets still floating around. The big Brittle Star is alive and well, but is affected. I transferred him to the Hospital tank. Skimmer foam decreasing a little. It never overflowed. Water is still clear. Minimal stress to Acro’s thus far. Fish are still doing well.

Monday 07/29/24
12:26am. Dosed another 3mL. That’s 15mL total to my 100/G volume now. I will stop the dosing here. Changed Filter Cup wool for 2nd time. Tons of Worms, Pods, Limpets, etc. Fish still ok. Water is clear. Skimmer is ok. No bubbles in sump.

12:55am. Still the same. Corals look fine except for Goni still retracted. Some corals never lost PE. At this point I’m primarily seeing more dead smaller worms than anything. Fish are ok. Water is clear. Still no massive bubbles in the sump like others have reported. Skimmer operation is ok. I’m going to remove Power-filters, Siphon the Drain Chamber before the Filter Cup, check Overflow Boxes for any dead buildup, and siphon the tank one last time.

3:08am. One Yellow Tail Damsel is looking a little drunk in the flow. Definitely affected by medication. Other fish still look normal. Lowered skimmer down from 6” to 3” by taking out one of the skimmer stands (it’s been sitting on two stands). The amount of worms that died is mind boggling. They seem to keep coming in stages. More small worms than anything else. Limpet snails are completely dead. Corals look fine. Rainbow BTA looking normal. I didn’t use the mesh fish nets at all for collecting the die-off. I’m only Siphoning, running Power-Filters, and using the Filter Cup with wool to catch all the death coming down overflow to drain section. I siphon into the filter cup with return pumps on. So far the Siphon hose has been the best tool for clean up, with the Filter Cup being second. Power-Filters do well, but need to be wrapped thin/loose for good sucking power. They are better for smaller particles.

4:48am. I noticed a second Brittle Star finally get stunned enough to fall out from behind my inside Overflow Box. I relocated him to the Hospital tank. The Spionid worms are putting out slime, but I don’t see any hanging out of their dirt tubes yet or any that are dead. They’re completely retracted. I did see a white crab that I’ve never seen before. Apparently he wasn’t affected much, because he darted off quickly when he saw the flashlight. The Fish are still ok excluding the one Yellow Tail Damsel. I may need to remove him. Going to leave the medication in a little longer and go to sleep. No GAC or water changes yet. Corals look fine. Most have good PE, or ever better than before the treatment. A few maybe look slightly retracted.

6:00am. I’m gonna call it for now. I need to sleep a few hours, and then assess when to start GAC, and do a water change.

10:09am. Corals still look good. No color loss. Still have good PE. The Goni is the only coral that looks cranky with completely retracted polyps. Fish are all acting normal except for the one Yellow Tail Damsel is getting a little weak and looks drunk, but still able to swim ok. I don’t see any Spionid worms hanging out of their tubes or dead. Maybe 15mL to 100/G wasn’t enough, but it’s too early to call. I will turn on GAC now, and call this treatment done. It’s been 14 hrs. I’ll also perform a 20% water change shortly.

10:27am. I cut the lights off since I’ve had them on all night at about 20% trying to observe the tank. I’ll turn them on again at 1:30pm, and let them run to 9pm. Tomorrow I’ll resume a normal schedule and photo period. I removed my auto-feeder so the system will not be fed today. GAC is running with MJ-1200 feeding the BRS mini reactor.

11:00am. Performed 20% water change. Relocated drunk Yellow Tail Damsel to Hospital tank so that he doesn’t get sucked into a wave-maker. He is looking critical now.

3:40pm. Relocated a second Azure Damsel to the Hospital tank that was near bottom LT corner. The first Yellow Tail Damsel is looking extremely weak and barely swimming around despite no medication in Hospital tank. He’s bobbing around like a cork near the surface. Other fish in DT are doing ok so far, but I noticed they’re swimming more near the bottom of the tank which suggests they’re also feeling the effects of the medication. I do not believe this is an oxygenation issue now as I have my Deltec skimmer running, dual air-stones, dual return pumps. One of the power-filters is also breaking and rolling the surface of the water. Oxygenation and aeration are strong. This is more likely from overdosing Ivermectin. 150mg is a lot of medication.

4:52pm. Fish still acting a little weird. They are near the bottom of the tank. Will do another 30% water change. I haven’t replaced the GAC yet.

5:00pm. Tested KH, P, and N. P = 0.009 ppm, N = 10.1, KH = 8.8 (P < by 0.13 ppm, N < by 5.70 ppm, KH > by 0.3). The Phosphate plummeted with the water changes and medication combined.

6:29pm. Doubled PO4 dose. Turned down CaRx. The fish look more active after the 2nd water change, and swimming higher in the tank. The Goni still doesn’t look normal. Polyps are way less extended than usual. Looks very unhappy.

10:47pm. Hanna Phosphorus ULR test result = 0 ppm (wife tested). Had her double PO4 dose again. Currently dosing 0.12ppm Phos -N which is super rich and the bioavailable. I cannot afford to do any other water changes at this point until P starts to come up. So whatever medication is left in the tank will be staying there unless there’s an emergency. I’m a bit nervous at this point about the P. The wifey will test P here in a few hours. Hoping to get a value other than 0.

Tuesday 07/30/24
1:27am. P = 4 ppb or 0.012 ppm. Will test again when I get home around 7:30am, and tweak the dose if needed.

7:40am. P = 15 ppb or 0.046 ppm (> by 0.034 ppm). A small feather duster is flying around the tank. Apparently Ivermectin can kill those too. My 4 small JF Raja frags look incredible this morning. Better than I’ve ever seen them. All the eyes are very pronounced. Color is popping. However, the Goni still doesn’t look good. The remaining fish look better now. It’s been almost 24 hrs since the first Damsel went into the HT, and that fish is still as weak as it was when it first went in. This medication is obviously very harsh on some fish (we knew that already), and they don’t recover easily or quickly- even if removed from the medication. Both are still alive, but the first Damsel is still swimming vertically at surface, and the other has not moved. He is sitting on the bottom beside a PVC fitting. Both have labored breathing, are extremely weak, but are still alive.

8:24am N = 7.4 ppm (< by 2.70 ppm). Will hold here since P is low, and I expect a possible N spike soon, but we will see. I siphoned out everything previously, and siphoned the tank again this morning. I rechecked the drain and skimmer chambers. Tank is still fairly clean. I got most of the death.

8:46am. KH = 9.1 (> 0.3). Will turn down CaRx again.

4:50pm. P = 0.070 (> 0.02) N = 6.6 (< 0.80), KH = 8.8 (< 0.3). Ca = 430 (< 10), Mg = 1320 (< 16). Last values for Ca and Mg were on 07/17 from ICP results. Damsels are finally starting to recover. Corals look good, but not as good as normal due to the Water Changes, GAC, and crashing Phosphate. I’m unsure if the Ivermectin has a direct effect on Phosphate or if it’s because there’s a massive reduction of snails, worms, pods, or microorganisms contributing to the nutrient cycle. Obviously the water changes and GAC had an impact on N&P, but I don’t believe it would have dropped the P to zero. I was already dosing P prior to the treatment. This same effect happened to JCOLE (nutrients bottomed out). I’m dosing up Phosphate aggressively, but this could have easily crashed the system had I not taken action quickly. Definitely something to watch out for, but every system is different, and the opposite effect could happen where nutrients spike through the roof. I was prepared to act in either direction. I believe that is key when you’re doing crazy treatments like this. The glass is clean with very light tan biofilm when I pass the algae scraper, water is crystal clear from the GAC so for sure it’s working well. The system looks healthy and stable ATM, but it’s running too clean!

Wednesday 7/31/24 3:07am. I’m searching very carefully with a 300 lumen LED flashlight with all the lights off, and there’s no sign of the Spionid worms. None of their tentacles are out anywhere on the rocks or at the base of the corals. They slime at night or in the early morning, but there is no slime anymore. Nothing at all. I do see a lot of tubes with what appears to be a white/tan color worm hanging out, but not fully out, only like 1-2 cm. I think maybe they’re dead. Hard to say, but it’s looking promising. I’m honestly surprised, because I thought maybe the dose wasn’t potent enough to kill them. I’ll continue to monitor. One thing to note is that even though I nuked 95% of the Limpet population (which feels absolutely incredible to get all of those out of here), there are still about 5% that will survive as they climb out of the water in the moist drain section, climb up on the drain pipes, and I’ve also noticed some out of the water in the rear overflow box. However, a small 2-3mL dose later on should decimate the survivors if you push them into the water prior to treatment. It will take months or even a year for their numbers to ever get back anywhere close to where they were. There were at least 1,000+ Limpet snails in here prior to treatment. I also noticed that some worms did survive that were under my starboard. Not many though. So there could still be a few that are able to repopulate in the system. A second treatment later would likely wipe them all out. Dosed 5mL of CRT, and 1ppm of Nitrogen. Topped off my brute’s, and mixed up fresh water change water just to be safe.

11:10am KH = 9.0 (> 0.2), P = 0.162 (> 0.09), N = 8.3 (> 1.70).

Collected and sent OCEAMO ICP-MS to check if trace elements are rising. I expect a rise due to the medication obviously, but also from the instability of the Water Changes, GAC, and reduced KH consumption. Performed 20% water change after testing and collecting ICP sample. Replaced GAC. Goni was completely retracted and made me paranoid. That’s 70% of the water changed now. I do 20%, 30%, and now another 20%. All the other corals looking good, but some have a little less PE this morning. I see some pellets floating around also. It appears the fish aren’t eating. I will remove the feeder again. Dosed another 1 ppm of Nitrogen after the water change. Phos -N dose reduced to .08 ppm daily (< .04). Tested higher due to a.m. hours.

5:40pm. KH = 8.6 (< 0.40), P = 0.177 (< 0.05), N = 8.0 (< 0.30). GAC turned OFF! Tank running too clean with the water changes and fresh GAC. It’s just too much. PE is reduced or retracted on several Acro’s. I feel like the Water Changes and GAC are more harmful than the Ivermectin treatment itself! Water is clear, but you can definitely see the start of an imbalance in the tank after this last Water Change/ GAC change. The rocks and frag plugs are starting to display a brown color. Ostreopsis Dino’s are becoming more visible. On a positive note, the Spionid worms appear dead! Zero tentacles out. Even their dirt tubes look smaller and distorted. I cut some tubes off, and they haven’t rebuilt them yet. They’re usually quick to do that. Surprisingly, the second Azure Damsel died in the HT. He did not look that bad, and seemed to be doing well when I relocated him to the HT. He wasn’t that bad at all. The first Yellow Tail Damsel that I relocated was much worse, but seems to have recovered now. I fully expected the first Damsel to die, and this Azure to pull through. That was completely unexpected. Might be due to exposure time.?

Thursday 08/01/24 12:13am. KH = 8.6 (==), P = 0.159 (> 0.04), N = 8.8 (> 0.80).

7:11am. Fish are eating pellets again this morning, and looking healthy. Some corals still have moderate PE reduction. GAC remains OFF. The lack of PE has me a little nervous. I don’t like the system running this clean or with this much intervention. Corals are looking OK. None of the Acro’s are turning pale. So that is a good sign. I’ll avoid water changes for now, but will eventually restart GAC later on when the system fully recovers, and PE is back to normal. Goni is still retracted. Rainbow BTA looks normal. Very light tan biofilm on glass. The algae scraper is sliding smoothly. Nutrients are available. I dosed 1mL of Amino’s, 5 drops of Vitamins, 1mL of Liqui-Mud, and fed 5mL of CRT. Corals should pull through.

7:35am. KH = 8.8 (> 0.20), P = 0.132 (< 0.03), N = 8.6 (< 0.20), Ca = 430 (==), Mg = 1320 (==). Dosed 1 ppm Nitrogen. Phos -N increased back to 0.12 ppm daily. (This dry rock system loves higher P in the 0.15- 0.2+ range). I know that probably sounds crazy to many of you, but dry rock can be a different beast.

5:30pm. KH = 8.4 (< 0.40), P = 0.150 (> 0.20), N = 9.2 (> 0.60). Water crystal clear from the higher P dosing. Corals doing better. Still reduced PE on some Acro’s. GAC remains OFF. Goni still retracted and cranky. Ostreopsis Dino’s enjoying the destabilization. Fish doing well. Brown algae is developing on the rocks and plugs. Glass is relatively clean. There’s a very light tan biofilm. Shouldn’t take long for the tank to start to stabilize and balance again. Dosed 1 ppm of Nitrogen. Will keep Phos -N dose the same.

I’ll stop documenting for now, but will continue for my personal use as the tank comes back into balance. If anybody has questions, please feel free to reach out. If I do a 2nd treatment, I’d likely try about 5-10mL per 100/G, and start with 2-3mL. I just didn’t want to waste my time with a treatment like this, and not kill my intended target. That is why I dosed up to 15mL. At this point it appears that 15mL per 100/G will kill Coral Boring Spionid Worms, and about everything else without any problem’s. However, I think it’s likely excessive, and that a smaller dose will accomplish the same results. The question becomes how long to leave it in the system or when to do a water change.? Ivermectin seems to work in waves. It’s a very interesting medication. I’ll post the Pre/Post Aquabiomics Microbiome results soon. Will also share the ICP-MS I sent yesterday when that results this coming up week around Wednesday or Thursday (08-07 - 08-08).


 
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You finally got em!
I got them alright, but the ivermectin might have got all of my acro’s too! Situation getting a little critical now at day 6. I’ve continued to document though and I’ll post here later. Some corals loosing color. Did another 10% water change today, and turned down the whites. Trying to let the system take a break, but I’m not sure how much Ivermectin is still in the system, or how long it stays active. I’m sure it breaks down somewhat with the lights on, but I really don’t know. I’m unsure if some of the corals lost color from the initial high dose or if there’s still like 3mL active in here. So far I’ve changed 80% of the water now. They could just be loosing some color due to all the water changes, instability, nutrient fluctuations, etc. I’m just paranoid, because I don’t know if it’s due to the medication or the other. Biting my fingernails over here checking over every little thing, testing, and making sure there’s enough nutrients and nutrition going back in. There’s still moderate consumption so that’s encouraging. It’s funny because some corals lost color, but the TSA Fruity Pebbles had active growth this morning as I saw it burning the coralline algae off the rocks around the base. Go figure.
 
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Goni is super ticked. This is what has me thinking that a small amount of medication is still active and causing some corals to lose color. You can see why I’m a little nervous here.

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IMG_4575.jpeg
 

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I got them alright, but the ivermectin might have got all of my acro’s too! Situation getting a little critical now at day 6. I’ve continued to document though and I’ll post here later. Some corals loosing color. Did another 10% water change today, and turned down the whites. Trying to let the system take a break, but I’m not sure how much Ivermectin is still in the system, or how long it stays active. I’m sure it breaks down somewhat with the lights on, but I really don’t know. I’m unsure if some of the corals lost color from the initial high dose or if there’s still like 3mL active in here. So far I’ve changed 80% of the water now. They could just be loosing some color due to all the water changes, instability, nutrient fluctuations, etc. I’m just paranoid, because I don’t know if it’s due to the medication or the other. Biting my fingernails over here checking over every little thing, testing, and making sure there’s enough nutrients and nutrition going back in. There’s still moderate consumption so that’s encouraging. It’s funny because some corals lost color, but the TSA Fruity Pebbles had active growth this morning as I saw it burning the coralline algae off the rocks around the base. Go figure.
Current pics of the acros please..
 

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Oof. Did you test this in your display tank?

At least your limpet issue was solved.
 
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Oof. Did you test this in your display tank?

At least your limpet issue was solved.

Yes, but not a test. I had been dreaming of the treatment for quite some time fully aware of the risks involved.

If the tank crashes there is nobody to blame, but me. :)


IMG_5321_Original.jpeg
 
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Thank you for the documentation. Interesting to follow along. Actually a good read.

My pleasure. More to come. I’ll bounce back regardless of the outcome. Wasn’t happy with the worms in all the corals. When my joy was gone, it was time to take extreme measures.

Killing all the Limpet snails in itself would have made it all worth it. The Spionid worms were the icing on the cake.
 

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Yes, but not a test. I had been dreaming of the treatment for quite some time fully aware of the risks involved.

If the tank crashes there is nobody to blame, but me.
But then that means the cure was worse than the disease!

Did you lose any corals or are they just brown with lack of PE? I couldn’t read the whole thread because it was too long for me.
 

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Great read. Thanks for the documentation of this treatment. Huge risk, but you seem to have achieved your goals so far. Best of luck with the recovery. Can't wait to read the results from ICP and microbiome tests.
 
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But then that means the cure was worse than the disease!

Did you lose any corals or are they just brown with lack of PE? I couldn’t read the whole thread because it was too long for me.

Yeah, sometimes that can be the case. I haven’t lost any coral as of yet. Nothing has really browned out, but there is some color loss and PE reduction. Pale would be a nice word for some pieces. The water changes are just too much, but I want to make sure the medication is weak as possible or gone.

I observed 2-3 small bristle worms coming out from under the Starboard by the back glass as I was looking at the corals with my flashlight. So there are still a very small number of worms alive that can repopulate. This is also a good sign as the medication may not be very active or strong right now like I thought, because at 3mL of Ivermectin the worms started dying. The ARC Master Yoda appears to be the worst at this point. The entire base is pale, and it has these little white spots all over the base that almost look like little menstrual filament spots or something. Not sure if that is from stress or what. I saw the same thing on a few other corals yesterday, but it went away.
 
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Great read. Thanks for the documentation of this treatment. Huge risk, but you seem to have achieved your goals so far. Best of luck with the recovery. Can't wait to read the results from ICP and microbiome tests.

Thanks man. I knew it could crash the tank. I was thinking about it for a long time and considering everything that could go south. After reviewing previous Ivermectin treatments in the past I knew that somewhere around 15mL per 100/G would likely get them. I wanted to try a lower dose, but didn’t want to waste my time and effort only to see them pop up in a few weeks after treatment was completed. So I do not regret my decision with that dose, but at the same time I believe a lower dose will still get them. The questions remain the same. How long to leave it in? What is the minimal dose and time needed to kill them?How to remove it quickly? This all needs to be done w/o stressing the corals too much. I don’t think Carbon removes it very well which is why I’ve been doing more water changes.

As far as the ICP…I’ll screenshot my pre treatment results below. The sample was collected on 07/17, and treatment was started on 07/28. I collected another sample on Wednesday 07/31 before the water changes and dosing for that day. That will result this week around Wed or Thursday. I’ll also be sending two more ICP-MS about every two weeks to monitor trending and dial my dosing back in.

IMG_4476.jpeg


IMG_4477.jpeg



When the Aquabiomics results I’ll post that here as well. I do not expect very good results with this dry rock system. Although it will be interesting to see the post test results later and how the Ivermectin effected the Microbiome. The way it works is that you buy the 1st test. Collect the sample and send it in. Then he sends another test 4 weeks later and automatically bills you at that time for the 2nd test. So the post test results will not be here until the end of August or early September.

IMG_4591.jpeg
 
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Just wanted to share. These are not great pictures and of course at night with a LED flashlight, but you can see what I described previously as some kind of stress dots or menstrual filament’s that are dot-like in appearance. Color is white. Both corals have them. I’m unsure if the medication still in the system is irritating them, or if this was a feeding response, but I’ve never seen this when I’ve fed in the past. Just wanted to document it in case this happens in the future. Below you can see the white dots on both bases.


IMG_4590.jpeg



IMG_4589.jpeg
 
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I also want to link to the other thread that has both “Interceptor” and “Ivermectin” treatments/data in it so that these two threads will be connected and able to be found later:

 
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Here’s some interesting data I just ran across in a study. It appears that there is “rapid dissipation” of Ivermectin (3-5 days in water), but it can also bind to substrate.


“This paper describes the fate and effects of the anthelmintic drug ivermectin for a 265-day period following treatment (nominal concentration levels of 0, 30, 100, 1000 ng L(-1) (or parts per trillion (ppt)) in fifteen 12,000 L outdoor aquatic mesocosms. Although it is established that ivermectin is highly toxic towards invertebrates, it has been believed that ivermectin does not present notable risks to aquatic systems due to the rapid dissipation of the compound and binding to the sediment. Hence, fate and exchange of ivermectin between water and sediment were evaluated in this study. The ivermectin DT(50aqueous) in water was found to be 3-5 days, but concentrations increased and appeared to be stabile in the sediment at 20-30 ng kg(-1) with no assessable DT(50sed). Acute effects (first week) following ivermectin exposure were identified and cladocerans were particularly sensitive (nom. 100 ppt). Chronic responses (<day 97) were observed for the ecosystem structure and function (nom. 30 ppt). Long-term effects (>229 days) were identified for more sediment-active organisms (e.g. Chydoriae and Ephemeroptera) (nom. 1000 ppt). This is the first study to demonstrate the potential environmental risk of ivermectin at or below the predicted environmental concentration using a standardized test methodology (mesocosm) with minimal extrapolation uncertainty.”
 
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Reefahholic

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This study seems to suggest otherwise:

Distribution of Ivermectin in the water and sediment​

The concentration curve of IVM in the water is shown in Fig. 3. The concentration of IVM was 0.092 ng mL−1 at 0.5 h after oral administration. It decreased to 0.084 ng mL−1 one day later. The concentration reached its peak at 7 d (0.113 ng mL−1) and 30 d (0.115 ng mL−1). The concentration of IVM then gradually declined and reached a value of 0.076 ng mL−1 at 70 d.


IMG_4600.jpeg


Figure 3
IVM concentrations in the water.
The curve indicates the change of IVM concentrations in the water from 0.5 h to 70 d after the single oral administration.



The concentration of IVM in the sediment reached 3.141 ng g−1 at 0.5 h and accumulated continuously to its peak of 3.863 ng g−1 at 30 d. After that, the IVM concentration in the sediment also gradually declined and reached a value of 2.946 ng mL−1 at 70 d, which was similar to the trend with IVM in the water (Fig. 4).


IMG_4601.jpeg



Figure 4
IVM concentrations in the sediment.
The curve indicates the change of IVM concentrations in the sediment from 0.5 h to 70 d after the single oral administration.




Distribution of IVM in the aquatic environment​

In this study, the reason that the concentration of IVM in the sediment was higher than that in the water should be due to the hydrophobic properties of IVM and its high affinity to organic matter (Bloom & Matheson, 1993). After treatment, the IVM was quickly detected in both the water and sediment. This is consistent with reports that the IVM could rapidly diffuse from the water phase to the sediment particles (Löffler et al., 2005).

In addition, two concentration peaks of IVM appeared in the water and sediment. First, it appeared at 0.5 h after treatment. When the drug was given, part was immediately excreted by the gut to the aquatic environment for the nervous swimming of the fish and their gut cleaning. It was similar to the report that only 30% of IVM orally administered in salmon was measured in the muscle, blood, kidney and liver (Høy, Horsberg & Nafstad, 1990). After that, the second peak appeared at 30 d in both water and sediment, followed by a decline in the concentration of IVM in the aquatic organisms (including fish, mudsnails and plants). Thus, we deduced that both the water and sediment were crucial agents for the transfer of IVMs in aquatic system. In addition, IVM accumulated in the aquatic environment for a long time.
 
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