First off i like to apologize for not doing any updates, I was busy through the holidays. Anyways i have updates: I ran second and third round of study on the 4 bottles. 1. Bio Spira 2. Microbe Lift Nite out 2 3. Atm Colony 4. Fluval cycle I dosed each tank with 1 ppm of ammonium chloride. Following the manufacturer recommendation i dosed bacteria accordingly 1. Bio Spira: 16.5 ml per 5 gal once 2. Microbe Lift Nite out 2: 2.5 ml per 5 gal every other day 3. ATM Colony: 47.2 ml per 5 gal once 4. Fluval Cycle: 12.5 ml per 5 gal 1st day, 5 ml 2nd day and 5 ml 3rd day. On the Side study I had 2 tanks setup and dosed 1 tank with Fritz turbostart 900 kept in fridge and other with Turbostart 900 left at room temp for over 1 month now. Along with the Side study came the @brandon429 study as well. I will post results of everything in the next post.