I'm a bit lost with my Trident readings after changing Reagent B & C. My Calcium and Magnesium levels have been really steady and consistent for the last couple of months. Calcium right around 450. I changed out the reagents last night and suddenly my Calcium is reading around 290. I've run the setup a couple of times to make sure the lines are properly primed. I made sure to shake the reagent bottles. Any other ideas?