also must add: I gotta see that nh3 totally stuck on a digital meter to be 100% bought in its dead. ya'lls known api skills have me at 99.9% tho
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Or you could have a contained environment outside of the display like Donovan's Nitrate Destroyer.(If I had high NO3, I would want anaerobic space where denitrification might be accelerated)
I have an odd respect for your persistence with an idea that no one else in the hobby thinks is important (nitrification in the water after a cycle).wonder if suspension cycling (swirling suspended unattached nitrifiers destined to be sinked or skimmed) is helping the boost and removal at the faster rate, will a water change even out the performance
wonder if suspension cycling (swirling suspended unattached nitrifiers destined to be sinked or skimmed) is helping the boost and removal at the faster rate, will a water change even out the performance
ammonia added to one of the cycled tanks.I got you covered. I'm pulling a 100mL sample of just water and another sample of water + 2% sand from a cycled tank. We'll see if the water is active, or if all the activity immediately after a cycle is in the sand.
A single dose of biospira + worst possible live rock + dry sand + 9 day cycle = more than 10x the nitrification rate of my 10yr old sand bed.
A point of interest. The biofilm that forms on glass slides in my system after 15 days do not oxidize ammonia but coat glass slides with aragonite and in five days the glass-aragonite is oxidizing ammonia. That is old news but points out that sand or maybe any rough surface can quickly be recolonized with ammonia oxidizing bacteria. Good news for “rip-cleaners”, right @brandon429 ? The point of interest is those AOB seem to be getting into the water either individually or as pieces of biofilm and rendering the water a weak ammonia oxidizer. Will that explanation work?So here's the results to this
ammonia added to one of the cycled tanks.
100mL of water-only in one sample
100mL of water with 2% (2mL) sand in the other sample.
samples on orbital shaker at 70rpm in the dark...
The sample with the newly cycled sand is processing at a rate of ~2ppm ammonia-nitrogen per day (about the same rate as the cycled tank the sample was pulled from).
The water only - less than 1/10 of that, ~0.15 ppm per day. It's probably not zero, but I can't really nail it down 100% because the water was filled with NO2/NO3 before I started, so I can be definitive and show the corresponding increase in NO2/NO3.)
I can say from this that no one should ever worry about losing nitrification capacity from doing a 90% water change after a completed cycle, you would lose an un-noticeable amount of nitrification.
(Unless you cycled with a product that isn't a chemoautotrophic nitrifier. Then all bets are off. Those heterotrophs could be anywhere.)
This also ties up the loose end for me: That the protocol I used (a few % sand in 100mL jar on an orbital shaker at 70rpm) is actually capable of detecting high nitrification rates of several ppm per day.
So those samples from my tank and the LFS that only nitrified a couple of tenths ppm per day, they simply didn't have many nitrifiers.
A single dose of biospira + worst possible live rock + dry sand + 9 day cycle = more than 10x the nitrification rate of my 10yr old sand bed.
I wonder whether the old sand bed has the amount of ammonia oxidizing capability it does because the organic amines and ammonia are being processed differently than in a new system. Said another way, the new aquarium starts out in an inorganic nutrient rich regime and moves to an organic nutrient rich one. Nitrification dominates early but then declines in importance and capability as the system ages and depends more on heterotrophic bacteria. The state of the nutrient regime might also have an influence on how biofilms mature and which nuisance photosynthesizers thrive in the system’s biofilm.So here's the results to this
ammonia added to one of the cycled tanks.
100mL of water-only in one sample
100mL of water with 2% (2mL) sand in the other sample.
samples on orbital shaker at 70rpm in the dark...
The sample with the newly cycled sand is processing at a rate of ~2ppm ammonia-nitrogen per day (about the same rate as the cycled tank the sample was pulled from).
The water only - less than 1/10 of that, ~0.15 ppm per day. It's probably not zero, but I can't really nail it down 100% because the water was filled with NO2/NO3 before I started, so I can be definitive and show the corresponding increase in NO2/NO3.)
I can say from this that no one should ever worry about losing nitrification capacity from doing a 90% water change after a completed cycle, you would lose an un-noticeable amount of nitrification.
(Unless you cycled with a product that isn't a chemoautotrophic nitrifier. Then all bets are off. Those heterotrophs could be anywhere.)
This also ties up the loose end for me: That the protocol I used (a few % sand in 100mL jar on an orbital shaker at 70rpm) is actually capable of detecting high nitrification rates of several ppm per day.
So those samples from my tank and the LFS that only nitrified a couple of tenths ppm per day, they simply didn't have many nitrifiers.
A single dose of biospira + worst possible live rock + dry sand + 9 day cycle = more than 10x the nitrification rate of my 10yr old sand bed.
That's pretty nice that you were able to catch AOB colonizing aragonite from the water in just a few days when they were unable to colonize glass in the same conditions.A point of interest. The biofilm that forms on glass slides in my system after 15 days do not oxidize ammonia but coat glass slides with aragonite and in five days the glass-aragonite is oxidizing ammonia. That is old news but points out that sand or maybe any rough surface can quickly be recolonized with ammonia oxidizing bacteria. Good news for “rip-cleaners”, right @brandon429 ? The point of interest is those AOB seem to be getting into the water either individually or as pieces of biofilm and rendering the water a weak ammonia oxidizer. Will that explanation work?
I'm not totally convinced that every system eventually settles to a low-substrate-nitrification equilibrium over time (Maybe just every system I get my hands on). Aquabiomics does find some systems that (at least as far as genetic sequences go) look like they possess a lot of nitrification. The BattleCorals system (article) was one of those.Said another way, the new aquarium starts out in an inorganic nutrient rich regime and moves to an organic nutrient rich one. Nitrification dominates early but then declines in importance and capability as the system ages and depends more on heterotrophic bacteria. The state of the nutrient regime might also have an influence on how biofilms mature and which nuisance photosynthesizers thrive in the system’s biofilm.
I agree. Unfortunately, we know virtually nothing about the systems Aquabiomics tests and finds or doesn’t finds nitrifyers. Hard to know if the information is important or a so what.I'm not totally convinced that every system eventually settles to a low-substrate-nitrification equilibrium over time (Maybe just every system I get my hands on). Aquabiomics does find some systems that (at least as far as genetic sequences go) look like they possess a lot of nitrification. The BattleCorals system (article) was one of those.
"Ammonia-oxidizing microbes made up almost 8% of your sample, which is higher than about 85% of aquariums I’ve tested, about twice as high as the average sample. Your sample also had high levels of nitrite-oxidizing bacteria (0.9%), which is among the highest of any tanks tested (higher than 96% of samples), and over 3-times higher than the average sample."
It's not a chemical test result, but a nearly 1% population of Nitrite Oxidizers is pretty strong evidence that a good amount of ammonia is not assimilated but is being processed through nitrification.
It's only one data point, but it's a tantalizing one, that a clearly desirable, coral-dominated system would have one of the highest measured nitrifying communities.
Neat experiment! Curious why the bio Spira was added? While low quality, the rock was live. Any testing done for nitrate?Each tank got Instant Ocean water, 500mL Caribsea aragonite dry sand, and low quality "live" rock from Petco. (The rocks had all been kept in a pile in the same tank. I imagine they are pretty similar). Each tank got the minimum recommended dose of Biospira, and were left to circulate with the lights off.
With regards to your system. Have you added any livestock recently? Or anything at all that could of brought in some goodies!I'm not totally convinced that every system eventually settles to a low-substrate-nitrification equilibrium over time
I am not an academic but kind of feel like this might be what it’s like to hone in on a dissertation topic.
Anyhow, if only R2R awarded PhD’s.
This is fantastic reading. Thank you @taricha for doing actual research and pushing on questions that are central to how we think about N in reef aquariums. This gives me a lot to think about in managing my own box of saltwater.
Two reasons.Neat experiment! Curious why the bio Spira was added? While low quality, the rock was live. Any testing done for nitrate?
Not sure what livestock you are thinking that could push the nitrifier population way down.With regards to your system. Have you added any livestock recently? Or anything at all that could of brought in some goodies!
Good point about activity from Biospira and not much from rock. If the typical live rock is like your “lazy sand”, the hobby might be overestimating the capacity of live rock to oxidize ammonia though it would be a excellent source of bacteria as Aquabiomics has demonstrated.Two reasons.
One, I wanted to replicate a low effort typical starting condition that many hobby tanks might experience.
Two, in reading through an aquabiomics article I was a little taken aback at how slow the nitrification response was from "maricultured live rock". I'll dig up this data later, but it leads me to believe the majority of the activity I saw in the first two weeks is from the Biospira bottle and maybe not the rock.
Not sure what livestock you are thinking that could push the nitrifier population way down.
Algae and coral as consumers of ammonia seems to me a plenty sufficient explanation for the low nitrification activity of substrate.
Two reasons.
One, I wanted to replicate a low effort typical starting condition that many hobby tanks might experience.
Two, in reading through an aquabiomics article I was a little taken aback at how slow the nitrification response was from "maricultured live rock". I'll dig up this data later, but it leads me to believe the majority of the activity I saw in the first two weeks is from the Biospira bottle and maybe not the rock.