Experimenting with in-tank antibiotic treatments for Brown Jelly Disease

BRS
Brown Jelly Disease (BJD) is an issue many reefers have run into. Like most coral diseases, the pathogen causing BJD has not been conclusively determined. Here I will share some observations and test results supporting the idea that its caused by a bacterial pathogen, and so may be treatable with antibiotics.

I'm sure this is not the first time someone's tried antibiotics against BJD, but I havent previously come across a DNA based study of the results. This is a preliminary investigation based on observations made on my home tanks spanning a few months. It is not conclusive, and is not intended to put the discussion to rest; far from it. It's just another piece of the puzzle as we figure out how to deal with this disease.

Overall my finding suggest that:
  • Infection with a specific bacterium in the genus Arcobacter contributes to BJD.
  • This infection can be safely treated in the aquarium with low doses of a commonly used aquarium antibiotic.
  • This treatment doesn't appear to damage the microbial community, but may actually improve it.
Here is the story, including the single case study so far of a tank treated in this way.

A Specific Bacterium Associated with Brown Jelly Disease
Over the last 6 months I've seen several cases where newly imported Euphyllia developed Brown Jelly Disease shortly after I received them. It had all the signs of an infectious disease. A newly imported colony would dissolve in BJD, and several other frags Euphyllia in the tank would also develop BJD if I didn't remove the affected corals quickly enough.

These corals aren't cheap, so my instinct was initially to try to save them (with no success) rather than conduct experiments. But after losing a few Euphyllia from different orders and suppliers my curiosity got the better of me. So the next two corals that developed early signs of BJD (a Hammer Coral E. ancora, and a Grape Coral E. cristata), I let it develop. Once they were in full-blown BJD, I sacrificed and sampled them.

Both of these samples were dominated by a single type, an unclassified bacterium in the genus Arcobacter. To clarify what I mean by that: this is a type that has been seen before, it's a perfect match to a sequence record in public DNA sequence databases. But, like so many microbes found in environmental samples, the species has not been formally described and named.

BJD pies.jpg


Identifying it to the genus can be pretty useful, though. The genus Arcobacter includes several known pathogens of humans and other animals, including A. butzleri, which is a food-borne pathogen that presents a serious risk to human health. When you sequence samples of two different diseased corals, and find them both dominated by a genus known to contain pathogens, it's worth noting.

It also gives us a good guess at the likely physical description and lifestyle. These are rod-shaped to helical, non-spore forming, and swim in a corkscrew motion using their single flagellum. These occur in association with animals, and also free living in waste water, seawater, and other aquatic habitats.

This information can also be useful in developing treatment strategies, since the members of a Bacterial genus often share characteristic sensitivities to the same antibiotics. We'll get to that.

Infecting a Euphyllia Garden with BJD
Who would intentionally do such a thing?? Not me.

What happened was this. I continued to buy Euphyllia, a fraction of them continued to develop BJD, and I was experimenting with short antibiotic dips. (This approach never yielded much success for me, but there is more work to be done on that front. )

Then one batch of especially nice corals started to BJD within 24 hours after arrival, and I panicked. I decided my Frag Tank must have the BJD pathogen, and I needed to get them out of it. I triaged the new corals, sacrificing the worst cases and transferring a couple that I thought were clean into a different tank... my Euphyllia Display Tank.

This was not my smartest move ever. They were so pretty, I acted irrationally :(

Next thing you know, both of the new Euphyllia and 4 of my existing Euphyllia in the tank were showing early signs of stress. They all showed oddly deflated tentacles that were not fully extended, and I could the beginnings of some of the characteristic Brown Jelly stuff on the two new corals. I removed these plaguebearers, pretty though they were, from the tank.

So I sampled the water at this point... after introducing the new corals, at the earliest signs of stress. What I found: exactly the same bug (Acrobacter sp., sv1103) at unusually high levels. I found 92 sequences matching this perfectly, representing >1% of the total community. (These were never found in biofilm samples, this analysis is based only on water samples)
1607381633378.png

Treating BJD with antibiotics, in the aquarium
What to do? I could remove all the Euphyllia and try a dip, but I hadn't been having much success with dips. So I looked at the tank and realized, the only corals I cared about in this tank were the Euphyllia. Fish are easy to move to another tank if something goes wrong, I have plenty of tanks running with room for a fish or two.

So I decided to take the plunge and treat my whole tank with the antibiotic. Although it's often repeated on the internet that adding antibiotics to your tank will kill the good bacteria, I hadn't seen any evidence of that.

Meanwhile I had evidence an Arcobacter species was associated with BJD, published reports of the antibiotic sensitivity of these bugs, and a tank full of Euphyllia on the verge of succumbing to BJD. So I dosed the tank.

I chose Ciprofloxacin because it is effective at the lowest doses, reasoning that in this way, I could minimize collateral damage. I based this decision on this study because it had a nice comparison of dosage trials for a wide range of antibiotic; I saw several other studies that also supported the use of Ciprofloxacin to treat Arcobacter infections.

I used Ciprofloxacin at 0.125 mg / L. To achieve this I dissolved a 500 mg pill in 50 ml of RODI water, producing a 10 mg / ml solution (which I subsequently stored in the fridge). The aquarium system has 70 gal volume altogether (~265 L), so I added 3.3 ml of this solution to achieve 0.125 mg / L. I repeated this dose every 2 days for 3 doses altogether.

Within 24 hours of the first dose, all of the affected corals showed signs of improvement. Their tentacles inflated again, although the remained not full extended. Within 48 hours they were fully extended and looked perfectly healthy again.

A few days after the final dose, I sampled the aquarium again. Like samples taken from the same aquarium a month prior to introducing the diseased Euphyllia, Arcobacter sp. (sv1103) was again not detected. Introducing the diseased animals introduced the bacterium, and the antibiotic treatment appears to have knocked their populations down substantially.
1607384512881.png

I should emphasize that since there are no controls, or photographs, for the effects of this treatment on the corals it's entirely possible they simply got better on their own. We can have a little more confidence in the effects of the treatment on the Arcobacter themselves, though. Here we have a measurement of the effect, although not replicated in multiple tanks.

In a future experiment I will address both issues -- this was an experimental trial born of desperation.

But doesn't that kill the good bacteria too?
This is of course a reasonable concern. There's a wide range of views on how to achieve the right microbial community, and how much the various parts of the community even matter. But I think few experienced reefers would say the bacterial community doesnt matter at all. So it makes sense to be cautious about adding antibiotics to the tank and potentially killing off whatever parts of the community you think are important.

But of course the dose makes the poison, and remember that I chose this antibiotic specifically because it was effective against this genus at the lowest dosage of any antibiotics tested.

The system has a moderate bioload with 4 fish in a 40 gallon DT with 30 gallons of sump volume, along with a bunch of corals. There is no algal refugium or macroalgae. So if the nitrifying community were to die, I would expect to see an explosion in nutrients and algal growth. This didnt happen. (I confess, I didnt measure nutrients this time around. I base this conclusion on the lack of algal growth that would be fueled by a rise in nutrients)

More directly, I compared the aquarium microbiome before and after the treatment. Here's a table summarizing the major stats we look at in comparing aquarium microbial communities:
T-1 month
(before BJD)
T+1 day
(during BJD)
T+10 days
(after treatment)
Diversity Score (percentile)552 (91st)438 (69th)532 (87th)
Balance Score (percentile)0.5 (76th)0.18 (50th)0.51 (83rd)
Ammonia-oxidizing microbes (%)Present (5.5%)Present (2.3%)Present (0.9%)
Nitrite-oxidizing bacteria (%)Present (0.08%)Not detectedPresent (0.05%)

Did the treatment damage the microbial community? Not at all. If it had any effect at all, it was to restore the community to its previous state by selectively knocking out a few susceptible bacteria.

If all a person cares about is the nitrifying community, that was not removed. There was a slight decrease in AOM while NOB were restored from undetectable to a more typical level.

If a person is interested in the microbial functions of the community more broadly, the summary statistics of Diversity and Balance scores were also improved by the treatment rather than harmed. The introduction of diseased corals lowered both scores, and the antibiotic treatment increased both scores.

This point about the broader microbial community can be illustrated most easily with a barplot showing the community over time. In these plots, each color represents a different microbial family.

cipro community barplot.jpg

The figure shows that the microbial community was fairly typical before BJD, became atypical during BJD, and was restored to something like its previous state following the antibiotic treatment.

A skeptical reader may point out that since there are no replicates here, we can't be confident attributing the changes to the treatment. It could be coincidence. Perhaps the corals recovered on their own and the microbial community resumed its previous structure after a transient disturbance caused by adding diseased corals. Based on my previous efforts to save Euphyllia with BJD I find this a little unlikely, but certainly not impossible.

Ultimately that objection would be correct and in the future I will do a properly replicated experiment to test this. What I've shown here is a just hypothesis with some preliminary evidence supporting it.

How Common is this Bacterium in Saltwater Aquaria?
In a set of 148 aquariums I've sampled (this is not the entire database, its the "high quality" database made up of normal, healthy tanks with good sequencing coverage), Arcobacter sp. sv1103 is not detectable in a majority of tanks.
1607389031773.png

We can express this same idea a few different ways. For the majority oftanks, this sequence was not detected at all (80%). For nearly all tanks (95%), this bacterium was absent or extremely rare (<0.00006%).

This context makes the comparison with my BJD infected tank even more stark. That 1.2% is huge in comparison with the typical levels observed across a large number of aquaria.


Summary

Based on what I've reported here, I hypothesize that infections with Arcobacter sp. (sv 1103) contribute to Brown Jelly Disease in LPS corals, and that these infections can be safely and effectively treated in the aquarium with low doses of Ciprofloxacin. Depending on the results of further tests, this could be a useful tool for the hobbyist community in the fight against BJD.

Important disclaimer: if anyone reading this feels inspired to treat their own tank, I specifically take no responsibility for the results. This is an experimental treatment, and if you run experiments on your own tank you take all the responsibility for the results.

But I know some of you, like me, enjoy running experiments on your own tanks. Many of us have a bottle of Cipro sitting around. And anybody who keeps Euphyllia is likely to encounter BJD at some point. Anybody want to test this? I'll throw in the microbiome testing before and after if so...

Thanks for reading and may your tanks stay free of Brown Jelly!


[unless you're the kind of person inclined to run experiments with antibiotics on your tanks, so you can help me duplicate this :) ]
 
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Montagne

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@AquaBiomics could you please recommend an equivalent BJD “low dose” for enroflaxacin? It looks like the availability of cipro has recently changed.

The humblefish antibiotic directions for the two drugs incorporate differing bath times so it doesn’t seem like a good assumption to try and create a scale between the two. Thanks!

(Enrofloxacin available at https://everythingaquatic.net/product/enrofloxacin/)
 

shakacuz

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I don't do water changes after dosing and haven't seen any issues. Just the regular every other week or so usual wc.
trying to see the effects in a tank where no WC's were done after dosing/treatment.. i don't do WC's so i am extremely hesitant on doing any kind of treatment in my tank as i don't have room for a QT tank unfortunately.
 

Hans-Werner

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I haven't read the whole thread, just the opener, and I wanted to add just a comment: In my long-time-opinion phosphate limitation with nitrogen excess causes or at least favors BJD and is the root cause in most tanks.

Arcobacter was a key-word for a search and I made a find.

In general Arcobacter usually seems to be associated with pollution and specifically with feces.

My recommendation: Please check nutrient concentrations, especially phosphate, in tanks affected by BJD. Try stopping BJD by stopping dosing amino acids and maybe nitrate, increase phosphate concentration.

Watch and report, please. :)
 
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FREAKINRICAN69

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I haven't read the whole thread, just the opener, and I wanted to add just a comment: In my long-time-opinion phosphate limitation with nitrogen excess causes or at least favors BJD and is the root cause in most tanks.

Arcobacter was a key-word for a search and I made a find.

In general Arcobacter usually seems to be associated with pollution and specifically with feces.

My recommendation: Please check nutrient concentrations, especially phosphate, in tanks affected by BJD. Try stopping BJD by stopping dosing amino acids and maybe nitrate, increase phosphate concentration.

Watch and report, please. :)
So are you saying by dosing amino acids has to do with BJD or causes BJD or? Please advise as i'm trying to understand this whole ordeal and pardon my novice question.
 

Seachelle

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If anyone is having a hard time calculating how much they need to dose their tank with, I have attached the mathmatical work through. I also attached a picture of the cipro I used and where I purchased it from. You must always cross cancel your units when calculating this. That is how you will be sure that you did the math correctly, if you end up with the same units! Be precise and only round the final number.
Thank you for this! My brain was hurting trying to do the math!
 

KrisReef

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I didn't know this thread existed so now i have it bookmarked. I would like to prevent BJD from entering my tank, would Ciprio also treat and prevent? I plan on purchasing expensive torchs and protect my investment.

What's the update on the efficacy?
It’s been working for everyone I know for bjd without any issues with other things. It has been awhile since I used it so I don’t remember the dosage recommended/ used but I followed the instructions at the beginning of the thread and bjd went away. Can’t think of any reason to treat for bjd in a qt unless it is showing on a sick lps ( or treating an anemone?) it works quickly when it is needed but dosing prophylactic is how superbugs get developed. I wouldn’t do treatment for healthy animals.
 

Tired

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Isn't BJD thought to be nearly ubiquitous, on everything anyway? You'd probably have to sterilize everything wet you put into the tank, including fish- which isn't really viable.

Also, yes, please, /please/ do not increase antibiotic resistance by dipping seemingly healthy corals in antibiotics. Antibiotic use needs to be kept to an absolute minimum, because antibiotic-resistant bacteria can be incredibly dangerous. Not just to your system, to you and to everyone exposed to them.

It's not just a risk in your aquarium, either- antibiotics poured down the drain can build the resistance of the bacteria that encounter them, and genes from those bacteria can be passed from organism to organism, jumping across species. The same can happen if antibiotic-resistant bacteria from your aquarium encounter other bacteria somewhere else. The antibiotics themselves don't have to be present for the genes to be transferred.
 

MartinM

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It definitely works, but it shouldn’t be used as a prophylactic unless potentially in case of some kind of injury to the coral (rock fall, pump, etc). I’ve saved Goniopora from the infamous ‘flesh bubbling’ that happens to them with a higher dosage of this protocol (and also 24 hour high dose Amoxicillin baths).
 

woejillis

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I agree with what everyone else is saying. I don't use as a prophylactic.

When I see BJD in my tank, I take out the affected coral and treat it specifically with Cipro for 24 hours. At the same time, I treat my tank with Cipro, every other day for 3 days.

For me, it stops BJD immediately on the affected coral and prevents it from spreading.
 

Tired

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I agree with what everyone else is saying. I don't use as a prophylactic.

When I see BJD in my tank, I take out the affected coral and treat it specifically with Cipro for 24 hours. At the same time, I treat my tank with Cipro, every other day for 3 days.

Treating a tank-full of corals showing no symptoms is prophylactic treatment, and really not a great idea. For you to avoid most risk of antibiotic resistance developing, you'd have to use a dosage that would wipe out your entire biofilter. Any bacteria that are exposed to antibiotics and survive can develop genes to become resistant to antibiotics, and even harmless bacteria can pass those genes to pathogens.
 
BRS

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