@Eldredge Were you ever able to determine how long the phyto can survive once bottled and refrigerated?
Would be curious to know how much of the phyto is still alive after 1 month... 2 months, 3 months, etc... sitting in the fridge, being shook every day or two.
Right now I plan on dosing about 15ml daily to a my tank. doing so, should make a 32 oz bottle last about 2 months. Was just curious if the phyto would still be alive at that point.
Hello! Yes, I have stored a bottle in the fridge and checked it with a microscope periodically. The fresh live phyto will keep for quite a while in the fridge. As you said, it is best to give it a gentle shake once a day and open the bottle to let some fresh air in. For the first month, there really isn’t any noticeable change. During the second month, the motile cells become less active, but there is still a fair amount of activity. By the end of the third month, there isn’t really any activity, but the cells are still intact. If a new culture is started with the three-month old sample, it will grow and some of the cells will be motile. So, using the phyto within a month would be ideal, within two months is fine, and even three months is probably okay.