• For a Limited Time the R2R Partner Membership is NOW OPEN! Get some cool swag and chances to win part of over $20,000 in prizes! Click here for more details

Home brewed phytoplankton culture.

Bobby daniels

Community Member
View Badges
Joined
Oct 8, 2017
Messages
63
Reaction score
10
Ok in at a loss. I've been trying to culture my own phytoplankton for a few months now. The first batch I had great success with. I've tried 3 other times and my culture just dies. After about 3 to 4 days it just turns clear. The only thing that has changed since the first batch is its gotten a hell of allot hotter in my garage. Does phyto not grow after a certain temp. I've been trying to do this in my garage but with Oklahoma summers it does get around 100°. That's the only thing that makes any sense to me. The first pic is 3 days after the 2nd. Any help is greatly appreciated

20200809_172213.jpg 20200807_051919.jpg
 
Best reef aquarium LED lighting

Dilan Patel

Valuable Member
View Badges
Joined
Dec 25, 2016
Messages
2,258
Reaction score
1,678
Location
Nashville,TN
You can look at my thread called Phytoplankton experiment and I list all the steps ive taken! But from my understanding you need to lower that temp to around 74-80 degrees. Get some really good aeration and light it for about 18hrs. I dig a little more deeper into what ive tried and success and failures in the thread. I also think you should increase the f/2 not sure how much 2 cap fulls is ml wise.
 

jeffchapok

Well-Known Member
View Badges
Joined
Dec 31, 2018
Messages
858
Reaction score
1,156
Well I don't know how much 2 caps is, but I start off with about 4 ml of f2 per gal. If after 3 or 4 days it's not as dark as I'd like I'll add 2 more ml/gal and then let it run at least 2 more days. I keep this up until I get to the desired darkness. Usually within 8 - 10 days it's almost inky black.

IMG_20200626_180355293_HDR.jpg
 

Thespammailaccount

2500 Club Member
View Badges
Joined
Jan 3, 2020
Messages
2,670
Reaction score
6,628
Location
Houston
Was just having this conversation earlier with @TriggerFinger Phytoplankton is pretty hardy when it comes to temperature but I am not sure what the thermal threshold is to where it can negatively affect the culture. If it was not the heat it was more than likely some sort of contamination. Using water from your tank sharing equipment some airborne bacteria enter the culture from the pump to the lid can all cause a culture to crash. I run multiple cultures at once so there is a better chance that at least one culture makes it. I feel your pain I have been where your at have lost more cultures than I can count
 
OP
B

Bobby daniels

Community Member
View Badges
Joined
Oct 8, 2017
Messages
63
Reaction score
10
Just measured the cap, it's 5ml. So it's getting enough food. I did increase the photosynthesis time just now to 18hrs. I'll see if that helps. Thanks for the help everyone. Dilan Patel, I'll take a read thru your thread see if maybe I'm missing something.
 

jeffchapok

Well-Known Member
View Badges
Joined
Dec 31, 2018
Messages
858
Reaction score
1,156
I run my light 24x7. I also don't worry too much about contamination. I've even transferred tank water over just to see what happened (nothing). I also tried to hatch brine shrimp in one batch (they didn't live and the shells made a mess, but the phyto turned out okay).

Maybe I've just gotten lucky.
 

Silent

Valuable Member
View Badges
Joined
Feb 9, 2013
Messages
2,223
Reaction score
3,600
Location
Maryland
Phyto is such a basic life form it is almost hard to kill. Normally there is only 3 things that will keep you from being able to grow it and that is 1) contamination, 2) not enough light (amount and length), 3) not enough food.
Temp shouldn't be an issue because it will survive for a while in the fridge and it will reprodce faster in warmer temps.
My guess is the hot garage is causing them to repeoduce quicker and consume more food and are starving and dying off. Assuming #1 and #2 above are not an issue.
 

Do you have any "bully fish" in your tank?

  • YES (tell us in the thread)

    Votes: 20 48.8%
  • NO bullies just misunderstood...

    Votes: 20 48.8%
  • Other (please explain)

    Votes: 1 2.4%

Online statistics

Members online
1,897
Guests online
3,990
Total visitors
5,887

New Posts

Corals.com
Top