That is what Donovan and a few other aquarists figured out. The right sized media with the right sized denitrafier and with, the right flow and in an air tight and blacked out chamber is how they were getting anaerobic bacteria to grow. It’s way above my head but, I’m getting results and all inhabits still look happy.Yep, I’m familiar with how they are supposed to work as I was planning on using algae exudates from a turbo charged algae scrubber to be the carbon source, many moons ago. Just not sure the denitrification is taking place as suggested, in anaerobic conditions. It’s just my guess that a small open tank full of bioballs fed with aquarium water and a carbon source would do the same job, without all the faffing about. Obviously, wouldn’t be the first time I’ve been wrong though.