Measuring Salinity of Phytoplankton Culture

Fritz

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Well, I just answered my own question. I measured the salinity of greenwater with and without diluting, and the result was the same, so it looks like phyto in the water doesn't affect the reading- at least not significantly.
Great job. I've always wondered but never worried about it.
Now you've answered that question.

What kind of phyto are you growing?
 
AS

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Glad to help!

It’s just Nannochloropsis. I tried Iso, but couldn’t get it to grow.
yeah Iso is tricky and my nanno keeps invading it. I read somewhere it actually grows better if you co-grow brine with it. I know it likes to grow in my seahorse grow out tank. which is drilled so it has good flow and steady nutrients.

when I grow it in bottles it's a bust
 

taricha

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The algae cells do not affect the refraction gradient of saltwater, so no, the algae do not affect the readings of a refractometer.
nor do the neutrally buoyant algae cells change the density of the water, so a swing arm works fine too.
 
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yeah Iso is tricky and my nanno keeps invading it. I read somewhere it actually grows better if you co-grow brine with it. I know it likes to grow in my seahorse grow out tank. which is drilled so it has good flow and steady nutrients.

when I grow it in bottles it's a bust
Iso and T-Iso need extreme diligence in sterilization of culture water and equipment. Any sloppiness there and you will get contaminations.
 

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