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Message: <blockquote data-quote="ISpeakForTheSeas" data-source="post: 10633808" data-attributes="member: 185928">Agreed with Nano sapiens comment - sometimes it takes until a population booms, and sometimes the hitchhikers that haven’t been reproducing have simply been out of sight the whole time (there are tons of threads on here about fish disappearing even in small tanks for weeks on end only to reappear unexpectedly at a later date - if a fish can hide that effectively, I’d imagine smaller or more secretive creatures could potentially go unnoticed for months or even years).Also, as mentioned, dips do not remove all hitchhikers.Aside from frags and assuming the use of both dry rock and dry sand, new life could potentially come in on macroalgae, contaminated phyto or pod cultures, inverts of all kinds, contaminated water from livestock shipments, even on fish (though this one may be pretty unlikely for most, non-parasitic hitchhikers).Basically, if you want to prevent all hitchhikers (or at least as many as it is actually possible for you prevent, since we can’t really stop bacteria and viruses and other tiny organisms from entering our tanks), you would need to quarantine everything and fastidiously remove any hitchhikers you find/discard anything you find coming in with hitchhikers that you may not be able to remove.</blockquote>