Active MemberView Badges
- Jun 28, 2019
- Reaction score
What light intensity (was there light)? What water temperature? What were the beginning and ending levels of ammonia, nitrate and phosphate (and any other parameters you could provide)? Did you add any kind of organic carbon (if so, what kind(s) and how much)?
I reviewed my aquarium logbook for these details. In total, I had three five-gallon buckets cycling media, a 3 gallon QT tank and 10 Gallon QT tank cycling fir coral and fish respectively. Since I use a whole-house RO system for my water, I cannot make large batches. Instead my buckets and QT tanks were started over the course if a few days, letting the salt dissolve a full 24 hours before adding any media and bacteria. Each 5 gallon bucket held about 4 gallons of water and was started with 0.8-1.0 ml QuikCycl. The first two buckets got 7.5 ml TurboStart while bucket 3 got Dr Tim’s. Salinity was 1.025 and pH 8.2. Heaters ran around 78-80 degrees. Bucket 1 and bucket 3 just had an airstone for circulation while bucket 2 had a spare pump for circulation. Bucket 3 was in front of an east facing window, buckets 1 and 2 were next to a wall. I had a small light on low intensity blue for a while after adding coralline spores, but gave up on that after learning about their lifecycle in more depth. The QT tanks had fiji pink dry sand, dry rock, some coarse ARM reactor media as gravel, 78 degree heaters, led lights on a blue/white and HOB filter circulation. They were started with TurboStart and QuikCycle. After cycling, they were further dosed with MB7 and substrate sauce. QuikCycl was added to the buckets at a rate of 1 dr per gallon to maintain. This provided about 0.25 ppm ammonia per day. The QT tanks were fed by the inhabitants food. There has never really been measurable phosphate, even now in the DT. I started dosing nitrate and phosphate I had from SeaChem from my freshwater aquascape, but even a drop would tick off the corals, so I stopped.Awesome thanks.
Thanks again. No measured changes in NH4/NO2/NO3 and PO4 over that time period? Any way to guess the difference of temperature between buckets? How many buckets?
Yes, exactly, thank you! That is correct, they grow best with both light and organic carbon (photoheterotrophy in anaerobic environments), but can still eek out an existence with just light (e.g., photoautotrophy in anaerobic environments) or just carbon (regular ol' organoheterotrophy in dark aerobic environments). So long as they're growing, they of course remove ammonia and nitrate through assimilation. But I believe the high point of the product is its ability (Rhodopseudomonas, specifically) to perform denitrification. Its ability to degrade organic wastes is a close second, in my most humble opinion (obviously a big contrast with conventional nitrifiers). I feel very comfortable describing these microbes as 'cycling bacteria' owing to their ability to carry out both nitrogen fixation and (again, at least in the case of Rhodopseudomonas) denitrification. That being said, I prefer the term 'conditioning bacteria,' as that best describes their use in prepping new systems for the first big wave of stocking (i.e., big influx of organic wastes and nutrients, including nitrate); at that point, they'll typically have ample availability of light, nutrients and organic matter.Hey Kenneth my reading of the description for the PNS substrate sauce is that it's not meant to be / replace nitrifiers, but to supplement them. It also sounds from the description like you'd expect they wouldn't do much to ammonia if given no light or organic carbon. Is that about right?
I know, it's long overdue! I just tried to hook up an individual in Thailand via a possible new supplier in Taiwan, but they didn't want to ship that far. Apparently international to international distribution is pretty pricey nowadays (no surprise). DM me if you know of a good/big distributor in Thailand.