have a 300 gallon at office, everyone was fine at 6pm firday, sunday by 4pm, all but one fish dead! this is my boss's tank, it is well established, he has had most fish in it for over ten years... over $2k in loss. At a loss as to why ... is it possible the temp got too cold? there are no heaters on tank, never has been, the outsige temps never went below 55 for either nite, could 300 gallons go too cold that fast? Or could there be another issue? the tank has been there for years ... feel awful !! Please advise! ... apparently one fish survived, but lost 2 clarkie clowns, htppo fang, emperor angel, clown tfigger, yellow tang, black trigger, queen coris wrasse ... will get more details when get to work .... dont wanna go, itz gonna be so painful to see everyone gone ... should note, temps IN office not below probly 68 at very lowest, and hard to imagine even that low, days have been in the 70's ..