Extracting zooxanthellae from pulsing Xenia, anyone tried it?

[Liel_Uziahu]

Hello there,
I'm trying to extract and culture zooxanthellae. i know its possible.
I have large amounts of Pulsing Xenia, which has a good concertation of zooxanthellae.
Anyone tried it before? I've got some lab equipment for the process.
Does anyone know a regular microalgae (phytoplankton) grow medium can be used to grow them?

Cheers

​

 

[AdamD76]

you can buy some
Paper on growing it

 

[Rtaylor]

This article talks about how to separate zooxanthelle with stony coral. Might work for Xenia?

Zooxanthellae: Biology and Isolation for Scientific Study

The mutualistic symbiosis between corals and zooxanthellae is a well-known fact amongst aquarists. To improve our understanding of zooxanthellae biology, scientists isolate these symbionts from the coral host under a variety of environmental conditions. This article will provide an overview of...

reefs.com

 

[Liel_Uziahu]

you can buy some
Paper on growing it

where's the fun in that?
also i don't want a mixed of clades, i want to have different kinds

its for high school experiments, wanna do it with the kids

This article talks about how to separate zooxanthelle with stony coral. Might work for Xenia?

Zooxanthellae: Biology and Isolation for Scientific Study

The mutualistic symbiosis between corals and zooxanthellae is a well-known fact amongst aquarists. To improve our understanding of zooxanthellae biology, scientists isolate these symbionts from the coral host under a variety of environmental conditions. This article will provide an overview of...

reefs.com

im familiar with this article ,not think its gonna work since its not a stony
still gonna try it on monday

 

[taricha]

yeah, getting zooxanthellae out of any soft coral is super easy.
Cut off a tiny section and gently squeeze. you'll get drops of brown liquid come out. Each drop is uncountable zillions of the symbiodinium cells.
Never tried to culture them, but they are super-thoroughly studied, so I'm sure you can find the growth medium that they use.
I have done spectral stuff on them to look at pigment profiles.

 

[Liel_Uziahu]

yeah, getting zooxanthellae out of any soft coral is super easy.
Cut off a tiny section and gently squeeze. you'll get drops of brown liquid come out. Each drop is uncountable zillions of the symbiodinium cells.
Never tried to culture them, but they are super-thoroughly studied, so I'm sure you can find the growth medium that they use.
I have done spectral stuff on them to look at pigment profiles.

I'll give it a try on Monday

 

[Liel_Uziahu]

yeah, getting zooxanthellae out of any soft coral is super easy.
Cut off a tiny section and gently squeeze. you'll get drops of brown liquid come out. Each drop is uncountable zillions of the symbiodinium cells.
Never tried to culture them, but they are super-thoroughly studied, so I'm sure you can find the growth medium that they use.
I have done spectral stuff on them to look at pigment profiles.

i think ill try to blend some Xenia with a homogenizer, then centrifuge it to get a clean sample of zooxanthellae.
if it will work I will upload some pics here of the process.

 

[taricha]

Why add a bunch of tissue to be separated than just squeeze out liquid and centrifuge that?
(it'll probably work regardless)

 

[Liel_Uziahu]

Why add a bunch of tissue to be separated than just squeeze out liquid and centrifuge that?
(it'll probably work regardless)

need a large amount to start serval cultures

 

[Nate Chalk]

Why extract

 

[Liel_Uziahu]

Why extract

i want to try and grow zooxanthellae in large quantities for high school experiments. for example, recover bleached corals.