Growing Isochrysis with Glycerol

LordJoshaeus

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Hi everyone! I found this cool article on growing Isochrysis that may be helpful to those who need to culture microalgae for copepods, rotifers, and other live food critters; https://www.hindawi.com/journals/bmri/2013/983465/#discussion

Apparently Isochrysis galbana grows far better when small amounts of glycerol/glycerin (about 50 millimols, which is just under 3/4ths of a teaspoon per 2 liters of water) are added to the culture media, with algal dry mass in cultures being 2.1 times higher when cultured in that fashion than in a purely photosynthetic culture; they apparently are not the only algae that grow better when at least partially fed by a pre-made carbon source (the above article mentions several other examples). If I ever try to culture microalgae, I am going to try this :) I hope this helps you guys on your marine breeding endeavors.
 
I have an actual question for this thread! Would there be any benefit to harvesting Isochrysis (or any other microalgae) through a 1 micron filter sock and then placing the harvested algae in completely refreshed culture media (eg seawater with F/2 and glycerol)?
 
Did you ever have any luck with culturing iso?
 

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