Is this red stuff Cyanobacteria or Dino’s?

Dark_Knightt

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You're so lucky to have a microscope. That would help solve 99% of my algae issues. Well when I had cyano and other stuff. GHA is pretty easy to spot. Its green. And hair-like.
 

landlubber

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Pics look like cyano red mats, Dino are usually brown and snotty
while that's true in most cases it isn't that cut and dry which is part of the reason a microscope is a valuable tool. OP supplied photos which are invaluable and should be considered over a generalization of the usual appearance as who knows, OP could have both!
 

Dan_P

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Pics look like cyano red mats, Dino are usually brown and snotty
I agree on the ID based on color and film/slime appearance. It is the darn organism as @taricha points out that are not only what we did not expect to see but also do not give us clues as to what they are. The thick sheath that surrounds the organism might be a clue. Lots of things make snotty or slimey films and won’t help much.
 

Hans-Werner

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Obviously these are cyanobacteria of spherical shape (coccal), maybe Synechococcus or similar. I thought I knew a red coccal cyanobacterium but I don't recall the name.

Sometimes I find single cells of red coccal cyanobacteria when I examine sediment samples under the microscope but never forming mats yet.
 

Dan_P

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Obviously these are cyanobacteria of spherical shape (coccal), maybe Synechococcus or similar. I thought I knew a red coccal cyanobacterium but I don't recall the name.

Sometimes I find single cells of red coccal cyanobacteria when I examine sediment samples under the microscope but never forming mats yet.
@Hans-Werner thoughts on this one?

DB4D282A-3B9C-43C6-ADBD-2A6431D844AC.jpeg
 

vetteguy53081

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Cyano - no doubt
Cyano blooms typically start when water nutrient concentrations go haywire. Just like when you eat too much sugar and your waistline starts to bloom, the same happens in your tank when concentrations of phosphate, nitrate and other organic compounds are too high.
Some of the most common causes include:
- Protein skimmer which fills water with tiny air bubbles. As bubbles form from the reaction chamber, dissolved organic compound molecules stick to them. Foam forms at the surface of the water and is then transferred to a collection cup, where it rests as skimmate. When the protein skimmer does not output the best efficiency or you do not have the suitable protein skimmer to cover the tank, the air bubbles created by the skimmer might be insufficient. And this insufficiency of air bubbles can trigger the cyano to thrive.
- Overstocking / overfeeding, your aquarium with nutrients is often the culprit of a cyano bloom
- Adding live rock that isn’t completely cured which acts like a breeding ground for red slime algae
- If you don’t change your water with enough frequency, you’ll soon have a brightly colored red slime algae bloom. Regular water changes dilute nutrients that feed cyanobacteria and keeps your tank beautifully clear
- Using a water source with nitrates or phosphates is like rolling out the welcome mat for cyano. Tap water is an example
- Inadequate water flow, or movement, is a leading cause of cyano blooms. Slow moving water combined with excess dissolved nutrients is a recipe for pervasive red slime algae development

I recommend to reduce white light intensity or even turn them off for 5-7 days. Add liquid bacteria daily for a week during the day at 1.5ml per 10 gallons. Add Hydrogen peroxide at night at 1ml per 10 gallons. Add a pouch of chemipure Elite which will balance phos and nitrate and keep them in check.

After the week, add a few snails such as cerith, margarita, astrea and nassarius plus 6-8 blue leg hermits to take control.
 
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bark1ngspider

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I have a new tank 13.5g 1 month old with a pair of clowns and 1 emerald crab 3 astreas and 2 blue legged hermits and wondering if this algae is Dino or cyano or just new tank algae
 

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Uncle99

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Cyano - no doubt
Cyano blooms typically start when water nutrient concentrations go haywire. Just like when you eat too much sugar and your waistline starts to bloom, the same happens in your tank when concentrations of phosphate, nitrate and other organic compounds are too high.
Some of the most common causes include:
- Protein skimmer which fills water with tiny air bubbles. As bubbles form from the reaction chamber, dissolved organic compound molecules stick to them. Foam forms at the surface of the water and is then transferred to a collection cup, where it rests as skimmate
- Overstocking / overfeeding, your aquarium with nutrients is often the culprit of a cyano bloom
- Adding live rock that isn’t completely cured which acts like a breeding ground for red slime algae
- If you don’t change your water with enough frequency, you’ll soon have a brightly colored red slime algae bloom. Regular water changes dilute nutrients that feed cyanobacteria and keeps your tank beautifully clear
- Using a water source with nitrates or phosphates is like rolling out the welcome mat for cyano. Tap water is an example
- Inadequate water flow, or movement, is a leading cause of cyano blooms. Slow moving water combined with excess dissolved nutrients is a recipe for pervasive red slime algae development

I recommend to reduce white light intensity or even turn them off for 5-7 days. Add liquid bacteria daily for a week during the day at 1.5ml per 10 gallons. Add Hydrogen peroxide at night at 1ml per 10 gallons. Add a pouch of chemipure Elite which will balance phos and nitrate and keep them in check.

After the week, add a few snails such as cerith, margarita, astrea and nassarius plus 6-8 blue leg hermits to take control.
I followed this recipe for Cyano removal exactly.
Big difference in 4 days, gone in 10 days, sand is white and we are starting to slowly ramp the intensity back.
 

Dan_P

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Hello, I am having a takeover of this unknown organism. It was die to me using tap water to make my saltwater. I have now been using ro/di water for 3 weeks. 2 weeks now with a much higher quality salt.

I have taken some of the red stuff off and put it under a microscope. I can’t seem to identify if it is Cyanobacteria or dinoflagellates. It doesn’t look like either to me lol. Was expecting long stands of Cyanobacteria or the usual spikes of dinos. Any help would be fine. I assume the orange things are pennate diatoms.

Thank you!

756986DE-877F-4D43-A029-0B75BC254540.jpeg 5224DD1F-AC65-4E4A-8A59-8963D679AFE2.jpeg image.jpg
Try Haematococcus, alga.
 

flampton

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I agree on the ID based on color and film/slime appearance. It is the darn organism as @taricha points out that are not only what we did not expect to see but also do not give us clues as to what they are. The thick sheath that surrounds the organism might be a clue. Lots of things make snotty or slimey films and won’t help much.
Obviously these are cyanobacteria of spherical shape (coccal), maybe Synechococcus or similar. I thought I knew a red coccal cyanobacterium but I don't recall the name.

Sometimes I find single cells of red coccal cyanobacteria when I examine sediment samples under the microscope but never forming mats yet.

I'm not so sure you can just say obviously red cocci = cyanobacteria. They may be but from that pic there's no way I would make that proclamation.

So with that in mind I could also guess this could be a single cell rhodophyta, like Poryphridium sp.

What magnification is this? And with that knowledge what is the estimated diameter?

Anyways id culture them out. Don't know if you have the tools available to go that in depth though.
 

Dan_P

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I'm not so sure you can just say obviously red cocci = cyanobacteria. They may be but from that pic there's no way I would make that proclamation.

So with that in mind I could also guess this could be a single cell rhodophyta, like Poryphridium sp.

What magnification is this? And with that knowledge what is the estimated diameter?

Anyways id culture them out. Don't know if you have the tools available to go that in depth though.
How about Haematococcus?
 

flampton

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Haematococcus
Maybe? The main described species is freshwater, but apparently can grow in seawater in certain situations.

Also check Flintiella sanguinaria. Produces lots of exopolysaccharide. Brackish for that species but maybe another Flintiella sp. is marine.

You can try various staining techniques, also look for mitotic cells to see if you can see spindles, and/or test antibiotic sensitivities.
 

Hans-Werner

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How about Haematococcus?
Haematococcus are feshwater species. The red cysts are not slimy. Haematococcus is not wine red, more a clear red (German name "Blutregenalge", blood rain alga) or brick red.

The wine red color points to cyanobacteria or red algae. Both have the same pigments, phycobilins.
 

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Sure looks like a cyanobacteria. The structure changes alot from one species to the next but yours looks alot like this one.
 

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Dan_P

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Haematococcus are feshwater species. The red cysts are not slimy. Haematococcus is not wine red, more a clear red (German name "Blutregenalge", blood rain alga) or brick red.

The wine red color points to cyanobacteria or red algae. Both have the same pigments, phycobilins.
Great observations! Thank you.
 

taricha

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@Dan_P how much fun do you want to have?

For maximum fun, suck up a tiny amount of the stuff (pure is better) and do a cycle or two of freeze/thaw in plain water.
It'll turn the plain water pink - phycoerythrin, the pigment from cyano and red algae is water soluble.
(In the unlikely event it doesn't turn the water pink, then we're off base. That means it's not phycoerythrin and thus not a rhodophyte or cyano. Puts other wild cards from different lineage like haematococcus - red pigment is non-water soluble astaxanthin - back in play.)

Then take your pink water, filter or centrifuge, and run it in the spectrophotometer.
r-phycoerythrin from rhodophytes is spectrally different from c-phycoerythrin from cyanobacteria.

R-phycoerythrin - from red algae has a strong shoulder at a smidge under 500nm
R-PE.jpg



C-phycoerythrin (from cyanobacteria) has none or much less of a shoulder there in the 500nm ballpark.
The-UV-visible-absorbance-spectra-of-cyanobacterial-extracts-and-purified-pigments.png



I found that the difference shows up in spectra measurable from tank organisms
Red algae (gracilaria probably)
Gracilaria.png

(coralline algae has the same features - it's a red algae too).


and cyano from a purple photosynthetic sponge
Purple Sponge cyano.png

(red oscillatoria had almost the same features as here.)

In both cases, black lines are the measured spectra, and the other lines are me fitting pigments to match the observation.
 

Dan_P

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@Dan_P how much fun do you want to have?

For maximum fun, suck up a tiny amount of the stuff (pure is better) and do a cycle or two of freeze/thaw in plain water.
It'll turn the plain water pink - phycoerythrin, the pigment from cyano and red algae is water soluble.
(In the unlikely event it doesn't turn the water pink, then we're off base. That means it's not phycoerythrin and thus not a rhodophyte or cyano. Puts other wild cards from different lineage like haematococcus - red pigment is non-water soluble astaxanthin - back in play.)

Then take your pink water, filter or centrifuge, and run it in the spectrophotometer.
r-phycoerythrin from rhodophytes is spectrally different from c-phycoerythrin from cyanobacteria.

R-phycoerythrin - from red algae has a strong shoulder at a smidge under 500nm
R-PE.jpg



C-phycoerythrin (from cyanobacteria) has none or much less of a shoulder there in the 500nm ballpark.
The-UV-visible-absorbance-spectra-of-cyanobacterial-extracts-and-purified-pigments.png



I found that the difference shows up in spectra measurable from tank organisms
Red algae (gracilaria probably)
Gracilaria.png

(coralline algae has the same features - it's a red algae too).


and cyano from a purple photosynthetic sponge
Purple Sponge cyano.png

(red oscillatoria had almost the same features as here.)

In both cases, black lines are the measured spectra, and the other lines are me fitting pigments to match the observation.
You know that I hate fun :)

Your survey is very interesting. Obtaining a visible spectrum did cross my mind, but I am trying to avoid going down too many rabbit holes these days...but if this organisms takes off, I probably will give in to extracting pigments.
 

Jimbo662

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On Saturday I started the liquid bacteria / hydrogen peroxide treatment that @vetteguy53081 recommended. Tues afternoon I'd not seen any results and thought it wasn't working so I didn't dose the peroxide that night. By the next afternoon I was surprised by a change! I started seeing small round spots where the sand was beginning to clear. It seems to be clearing from the left side of the tank to the right. These pics were just taken. The tank started out with the sand completely covered like it is here in the front corner, it was always thicker to the front of the tank. The second pic is the left side of the tank. I was planning to do a water change tomorrow to try to remove as much as I can and see if the treatment keeps it at bay.
PXL_20240126_201254546.MP.jpg


PXL_20240126_201309914.MP.jpg
 

vetteguy53081

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Thi
On Saturday I started the liquid bacteria / hydrogen peroxide treatment that @vetteguy53081 recommended. Tues afternoon I'd not seen any results and thought it wasn't working so I didn't dose the peroxide that night. By the next afternoon I was surprised by a change! I started seeing small round spots where the sand was beginning to clear. It seems to be clearing from the left side of the tank to the right. These pics were just taken. The tank started out with the sand completely covered like it is here in the front corner, it was always thicker to the front of the tank. The second pic is the left side of the tank. I was planning to do a water change tomorrow to try to remove as much as I can and see if the treatment keeps it at bay.
PXL_20240126_201254546.MP.jpg


PXL_20240126_201309914.MP.jpg
This is cyano and make sure to follow what was mentioned precisely. Glad you’re seeing improvement
 

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