You can test by pushing your fingers into your sandbed until you touch the bottom glass. Then grabbing a handful of sand and picking it up a few inches off the sandbed, then release thru your fingers slowly.
If it clouds up a little in that area then youll want to rinse the sand. Remove as much sand as possible, place into buckets about half way full and rinse with a garden hose and jet sprayer until it comes out clear. After this, drain the tap water off and rinse once or twice more with 0ppm rodi. This will effectively remove all waste and not kill the beneficial bacteria thats encysted onto the sand.