I’m currently cycling my 90 gallon tank with dry rock. I tossed in some AF life source AF life bio fil and am also dosing microbactor 7 on the lighter side so I don’t overwhelm the tank with bacteria. I’ve put in 3 mysis cubes and 4 ocean plankton cubes in as an ammonia source and it’s been 5 days with still no sign of ammonia. Should I add more cubes or will it just take longer to see a spike?
If I assume a cube is 3 g, you added 21 g of material. Frozen food is mostly water, something like 80-90%. Just for this exercise assume 10% of the 21 g is dehydrated biomass or 2.1 g. Half of this is protein or 1.05 or let’s say 1 g. 16% of the protein is nitrogen or 160 mg N a bit more for ammonia, 200 mg. In 90 gallons or about 360 liters, that is a maximum of about 200 mg/360 L or about 0.5 ppm. This amount is not going to show all at once nor very quickly. I suppose it’s possible you will ever see ammonia if nitrifying bacteria are present. Testing for nitrite possibly gets around this issue becsuse it is more sensitive than the ammonia test and nitrite oxidizing bacteria can be slow to start (nitrite will accumulate).
You can dose a little ammonium chloride and watch for it to disappear to prove nitrifying bacteria are present.