Haven’t done it myself but I don’t see why it’s a problem.
I think I have decided to keep the air stone in the tank. I don't like it in the display, so I placed it in the first chamber that also holds my heater (which is currently not in use) Since I don't run a skimmer, I figured this would help put more oxygen in the water consistently. I have already seen an increase in PH, which is good. The middle chamber holds some bio media and the stock filtration sponge. Does any see any issues with running the air stone in the first chamber with this setup?