Culturing phytoplankton without fertilizer

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smhray

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Hey everyone, I was just wondering if it is possible to culture phytoplankton without fertilizer like f2 or other products. Also is there some other items I can use to fertilize? This is my first time trying to culture phytoplankton and I’m having trouble locating f2.
 
https://www.amazon.com/s?k=guillard...8&sprefix=guillard,aps,182&ref=nb_sb_ss_i_5_8
@andrewkw , I'm located in the States, any sources up in Canada?

And no. It's not possible to culture phytoplankton without fertilizer. The phytoplankton we work with are not diazotrophs (take in atmospheric N2 nitrogen), and thus need nitrogen supplementation as well as phosphorus, magnesium, potassium, and a couple of other things. You could try culturing with skimmate as a fertilizer, or better, miracle grow liquid fertilizer, but really Guillard's F/2 is highly recommended.
 

WHICH PART OF YOUR SETUP IS HARDEST TO REACH OR REMOVE FOR ROUTINE MAINTENANCE?

  • Glass behind or beside the tank

    Votes: 14 20.3%
  • Powerheads or wavemakers

    Votes: 5 7.2%
  • Sump or rear filtration chambers

    Votes: 9 13.0%
  • Skimmer or other filtration equipment

    Votes: 9 13.0%
  • Overflow or plumbing

    Votes: 12 17.4%
  • Lights or lids

    Votes: 0 0.0%
  • Mine is pretty easy to access!

    Votes: 19 27.5%
  • Other—tell us what I missed

    Votes: 1 1.4%
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