De-nitrifying bacteria. Substitute for Prodibio Biodigest?

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Hal3134

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I’m old school. Now that Prodibio Biodigest is getting hard to find in the 30 vial size, what do you recommend for reducing nitrite and nitrate? I still carbon dose but need the bacterial component.
 
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I think those bacteria come in with the air. As an old school reefer, I grab sand from a healthy mature system and inoculate both nitrification as well as denitrification bacteria.

Denitrifying bacteria reduce ammonia to nitrite and nitrite to nitrate in an oxygen environment. Facultative bacteria, in a reducing environment, break apart the nitrate molecule for the oxygen and release a free nitrogen molecule of gas.

PS: Nitrification bacteria double in population every 20 minutes. Denitrification bacteria take 7 hours to double in population.
 
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WHAT KIND OF SUBSTRATE ARE YOU RUNNING ON YOUR TANK?

  • Bare bottom

    Votes: 5 29.4%
  • A shallow bed of fine sand

    Votes: 3 17.6%
  • A shallow bed of coarser sand

    Votes: 3 17.6%
  • A deep sand bed

    Votes: 2 11.8%
  • Mixed substrate or varying sand depth

    Votes: 4 23.5%
  • Some other substrate (posted in the comments)

    Votes: 0 0.0%
  • I don’t have a reef tank yet

    Votes: 0 0.0%
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