i looked, but didn't find a dedicated forum for phyto... if it exists, someone point me to it!
I started a culture last night, but I am thinking I must have done something wrong. I bleached all equipment in a 10% solution for 10 mins. rinsed in RO water. then used alcohol to sterilize the jar, tube, lid, and bulkhead for the lid wearing nitrile gloves the entire time. I prepped a solution of 1 liter fresh made saltwater with .25ml of f/2 and boiled in an electric kettle. afterwards I put in sterile jar from before and put it in the fridge until it cooled off. in the meantime I put my jar of phyto on the counter (tetraselmis) to warm up some. in the end, I added 12oz of saltwater and fert, and 12oz of tetraselmis culture from TSA (who states its culture ready). today, the jar seems to have a film of green on the jar, that does not disperse if i agitate it some... i think my air pump was dialed too low, it was bubbling, but it seemed like the tetraselmis was not fully in motion in the jar, so i opened up the valve some.
I realize its probably newb stress, but hoping someone can tell me if i went wrong and how to correct? or if i need to just dump this round and try again?
also - anyone that is sterilizing via boiling, what do you do with the water while its cooling to keep it sterile?
thanks in advance. Im probably over-reacting...
I started a culture last night, but I am thinking I must have done something wrong. I bleached all equipment in a 10% solution for 10 mins. rinsed in RO water. then used alcohol to sterilize the jar, tube, lid, and bulkhead for the lid wearing nitrile gloves the entire time. I prepped a solution of 1 liter fresh made saltwater with .25ml of f/2 and boiled in an electric kettle. afterwards I put in sterile jar from before and put it in the fridge until it cooled off. in the meantime I put my jar of phyto on the counter (tetraselmis) to warm up some. in the end, I added 12oz of saltwater and fert, and 12oz of tetraselmis culture from TSA (who states its culture ready). today, the jar seems to have a film of green on the jar, that does not disperse if i agitate it some... i think my air pump was dialed too low, it was bubbling, but it seemed like the tetraselmis was not fully in motion in the jar, so i opened up the valve some.
I realize its probably newb stress, but hoping someone can tell me if i went wrong and how to correct? or if i need to just dump this round and try again?
also - anyone that is sterilizing via boiling, what do you do with the water while its cooling to keep it sterile?
thanks in advance. Im probably over-reacting...