Phyto questions

[hrdneglcry]

I have cultured phyto before. But this time I am using 500 mil jars. So like before I make the water just slightly green with phyto to start. Well normally it is said to use 5ml of food for one liter of water. So I added 25 ml of food because the jar is 5 liters. Is that ok?

 

[Blennioidei]

Most likely yes. I prefer not to over dillute, because when I did that, I got some yellowing rather it tourning green. It debends also on strain and fertilizer. But with pytho its pretty easy to decide if its done or not, and you can always add some more fertilizer

 

[afboundguy]

When I am doing my phyto I do 1000-1250 ml of fresh water and top off with the old culture to 1500 ml of that helps. The 1000-1250 depends on the strain as some of my 6-7 strains darken up much faster so I make them 1250ml of fresh SW vs others at 1000ml so they all get to roughly the same darkness around the same time so I can split them all at the same time.

 

[hrdneglcry]

I am trying to do phyto. I am including two pics. The ine liter bottles were from last time. I used the same procedure. You can see the green in the water. But this time it looks like the fertilizer has clouded the water. I used the same miracle gro. 5ml per one liter. But i am concerned that this isn't going to work. The current vessels are 5 liters. But the water looks very cloudy. Before when I added 5ml per the one liter it did not cloud the water. Is this even going to work this time?


Last time with one liters

This time with 5 liter bottles but see how the fertilizer clouded the water? It did not do this before.

 

[dansyr]

I am trying to do phyto. I am including two pics. The ine liter bottles were from last time. I used the same procedure. You can see the green in the water. But this time it looks like the fertilizer has clouded the water. I used the same miracle gro. 5ml per one liter. But i am concerned that this isn't going to work. The current vessels are 5 liters. But the water looks very cloudy. Before when I added 5ml per the one liter it did not cloud the water. Is this even going to work this time?


Last time with one liters

This time with 5 liter bottles but see how the fertilizer clouded the water? It did not do this before.

Looks like bacterial milkiness to me. A guess is that your inoculum (starter phyto) grew a little and then popped or popped immediately. That's carbon for the bacteria to eat, and the fertilizer is nutrients. Recommend draining, bleaching or autoclaving, and restart.

Kudos on the VWR bottles, that makes things easier :) they have rubber or butyl stoppers with two holes in them, that might make the airline situation easier than holes in caps. But this looks like it has great potential!

 

[hrdneglcry]

Maybe its what tou say but with the fertilizer. It did this as soon as I put in the miracle gro. Is this caput?

 

[BigAl07]

It looks like your culture didn't make it and now it's a bacteria habitat and going STRONG!

I would absolutely dump, clean, and restart. When in doubt, DUMP IT OUT!

I've never used Miracle Grow but I am curious as to what else they incorporate into their "formula" that may not be compatible to our systems. I only use Mercer F2 and have AMAZING results. My cultures turn such a deep green that one of my reefing buddies calls it "Green Cement Mix" which I'm never seen before LOL.

I start my cultures with a 50/50 culture to SW ratio and usually in 1 week it's fairly dark green and the next week it's DEEP green. I try to harvest at 2 weeks (split). I feed F2 at 50ml to liter. I feed upon splitting/starting and I feed again at the 1 week point. When I harvest, I don't feed until AFTER the split so that I'm not adding the new fertilizer to my harvested batch.

 

[dansyr]

Maybe its what tou say but with the fertilizer. It did this as soon as I put in the miracle gro. Is this caput?

This batch (with that color) is probably a goner. Milky color is almost always bacteria, lots of froth is often lots of broken open cells. Not sure how much froth I see looking at that (was confused with reflection of carpet on glass). but not great. Could give it a day or two to see if it bounces back, but that much turbidity for not being green or red is almost always non-photosynthetic bacteria (exceptions could be LOTS of fine particulates from something crashing out, you be judge of conditions may create that).

I've grown with miracle gro before (crystals I mixed up) but something like F/2 is definitely better long term. 5 ml/L miracle gro seems like a lot to me, but it totally depends how concentrated the miracle gro is you're putting in. My suggestion is that trying to do the math to figure out what concentration and forms of Nitrogen you're putting in for your specific miracle grow (and debugging based on what 'known' good concentrations are from F/2) is not going to be worth it, I would go trial and error - maybe try a batch with 1 ml per L from whatever miracle gro bottle you have, see how that goes. If it grows, great, next batch, try 2 mL per L and see if grows faster or darker or if it seems less happy. But remember could be a LOT of things - contamination, aeration too low?

 

[BigAl07]

It looks like your culture didn't make it and now it's a bacteria habitat and going STRONG!

I would absolutely dump, clean, and restart. When in doubt, DUMP IT OUT!

I've never used Miracle Grow but I am curious as to what else they incorporate into their "formula" that may not be compatible to our systems. I only use Mercer F2 and have AMAZING results. My cultures turn such a deep green that one of my reefing buddies calls it "Green Cement Mix" which I'm never seen before LOL.

I start my cultures with a 50/50 culture to SW ratio and usually in 1 week it's fairly dark green and the next week it's DEEP green. I try to harvest at 2 weeks (split). I feed F2 at 50ml to liter. I feed upon splitting/starting and I feed again at the 1 week point. When I harvest, I don't feed until AFTER the split so that I'm not adding the new fertilizer to my harvested batch.

That should read 1.5ml per Liter NOT 50ml.... I can't edit my original to correct that....

 

[hrdneglcry]

1.5 ml per liter not 5ml per liter? I'll wait a few days as I have not more phyto anyway. Maybe I'll but new Miracle Grow as it did not do this before. Unless I used to much. I could not find the original video I used last time. This new video I said 5ml per liter. So I used 5 ml per liter in 5 liters. It was green but as soon as I put the fertilizer in it was this color.

Its actually beginning to clear in one jar. I have two different lights and I think jar ones light is stronger. Its clearing andnI can see the green.

 

[hrdneglcry]

what happened was too mych fertilizer was innthe water and got the side of the glass hazy. this is the phyto today getting darker

 

[hrdneglcry]

This is what it looks like today as opposed to when I first posted. When do I have to pull it down and harvest it? How much darker should it be? Also there is a lot of fertilizer in there. 5ml per liter I used. How long will this be alright without harvesting?