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It's also a matter of what your refractometer is made for. There are ones for seawater, brewing, etc. I think I use a brewing one at the moment, but it was a gift bought off Amazon a decade ago so I'm not certain. If I calibrate to 0 with RO/DI it will be off by a couple ppt when reading in the 30's (according to the Red Sea one I inherited later). Just something I have to account for mentally. The slope of the readings is how they differ.