Just use all new sand
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Recently did a tank transfer as well, I did not use any old sand, but used the same live rock, same water, and dosed a small bottle of Dr. Tims nitrifying bacteria just to be safe. I had no losses.Question: I am transferring my existing 40 gallon reef tank to a 65 gallon and would like to use some of my old sand bed. What is the best way to do this? My plan is to only use the sand which I am able to thoroughly vacuum days beforehand and to ditch the rest. This leaves me with ~80% new sand to ~20% old sand. How would you go about combining them safely?