Phytoplankton Culture Issues

[FroggieJonnie]

Alright so I have 2 Cultures showing issues that I wanted to get some opinions on.

This was last night: I have just started the Rhodomonas culture a few days ago as well as just starting the T-Iso (right container with a barely yellow hue)

Today I get home and this is what I have:

Now lets give the photos credit where its due, in the picture I took last night I did have the back light off. So I can confirm a few things, the Green phyto is absolutely darker, and so is the rhodomonas.

Now to the situations im having and whats baffling me.

The T-Iso has for the second time just gone white. I dont understand why considering I was more confident about this culture doing well then I was the rhodomonas. Does this culture need to start off darker and more concentrated???

As for the rhodomonas, its not as red as I would have thought. More yellow / brown. Could this be a lighting issue?

Heres what I did:
I steralize the jars using rubbing alcohol on everything, glass, lid, tubing, connections, everything. Then I take fresh saltwater and boil it for a few minutes (I do wait until its boiling before timing it), dump it in the jar still hot, and let it cool. Once it hits room temperature I connect the tubing, put in around 3-4 ml of F/2, and add the start phyto. The saltwater starts around 1.022 salinity when I start boiling but I dont check after because honestly not that much water is missing once im done so I figure its close.

Any help would be loved and feel free to ask me question, I will answer to the best of my ability.

 

[That Crusso Kid]

Alright so I have 2 Cultures showing issues that I wanted to get some opinions on.

This was last night: I have just started the Rhodomonas culture a few days ago as well as just starting the T-Iso (right container with a barely yellow hue)

Today I get home and this is what I have:

Now lets give the photos credit where its due, in the picture I took last night I did have the back light off. So I can confirm a few things, the Green phyto is absolutely darker, and so is the rhodomonas.

Now to the situations im having and whats baffling me.

The T-Iso has for the second time just gone white. I dont understand why considering I was more confident about this culture doing well then I was the rhodomonas. Does this culture need to start off darker and more concentrated???

As for the rhodomonas, its not as red as I would have thought. More yellow / brown. Could this be a lighting issue?

Heres what I did:
I steralize the jars using rubbing alcohol on everything, glass, lid, tubing, connections, everything. Then I take fresh saltwater and boil it for a few minutes (I do wait until its boiling before timing it), dump it in the jar still hot, and let it cool. Once it hits room temperature I connect the tubing, put in around 3-4 ml of F/2, and add the start phyto. The saltwater starts around 1.022 salinity when I start boiling but I dont check after because honestly not that much water is missing once im done so I figure its close.

Any help would be loved and feel free to ask me question, I will answer to the best of my ability.

I didn't even read your full post but it appears you're having Phyto issues. I am tagging my friend, @TheNative192 because he is good with Phyto.

 

[TheNative192]

Looks like you definitely need to start with a higher culture concentration for sure. I would add more F2 Fertilizer as well to see if it can recover. Think about is as fertilizer for a plant. If you have a small plant and want it to grow to a large plant, it will take more time and fertilizer, if you start with a medium plant it will take less time and less fertilizer to grow into a large plant.

Same thing applies here.

Also is there enough light? Is light only coming from the left side or is it on both sides. Do you run the lights 24/7 or 12-16 hours. Also how much water movement is there. A video would help. Some cultures are also a lot harder to maintain.

The three things I would say is:

1. Too small of starter culture,
2. Not enough light or 24/7 light is bad (get a cheap timer plug on amazon for $5
3. Not enough water movement.