This may be a bit in the weeds but I have a blended live phyto culture of Tetraselmis and Nannochloropsis. As an experiment, I want to see if I can isolate the Tetraselmis via hetertrophy. My understanding is that Tet. reacts quite positively to heterotrophic culture methods while Nanno does not.
I was reading this paper and the section describing the heterotrophic method, it mentions that the culture was acclimated from a 24hr light schedule to strict heterotrophy by gradually decreasing the light schedule. My question is whether this is actually necessary. Is this something that I should do or can I just try putting the jar in the dark with an air pump?
I was reading this paper and the section describing the heterotrophic method, it mentions that the culture was acclimated from a 24hr light schedule to strict heterotrophy by gradually decreasing the light schedule. My question is whether this is actually necessary. Is this something that I should do or can I just try putting the jar in the dark with an air pump?
The adaptation of an autotrophic to a heterotrophic culture of T. suecica was carried out in 100-mL glass photobioreactors with a diameter of 3.0 cm and constant temperature (21 °C). A batch culture system was started. The reduction of the photoperiod illumination time was progressive (light:dark) 12:12 (288h), 8:16 (228h), 4:20 (240h), 3:21 (156h), 2:22 (168h) and 0:24.