That’s exactly what I did last night actually. I calibrated it with the 10 day old opened solution.
Alk 8.1 calcium 425 mag 1255 as per solution label. Confirmed these number after calibration.
Ran a combined test sequence after the calibration was done.
Alk 9.0 calcium 429 mag 1474
I measured alk with my Hanna, calcium with Salifert, I don’t have a mag test kit but I was pretty sure I’m in the mid 1400s
Alk 9.0 calcium 420, mag no kit to verify.
So confirmed, the solution remained extremely accurate to calibrate the trident after 10 days. By the amount used for a calibration, I think you can get 3 uses per bottle. Minimum 2 for sure.