Brooklynella

Discussion in 'Fish Disease Treatment and Diagnosis' started by Humblefish, May 8, 2016.

  1. Humblefish

    Humblefish Valuable Member R2R Supporter R2R Excellence Award Article Contributor Expert Contributor Moderator Emeritus

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    Brooklynella hostilis

    What You Need To Know:

    * This is most often seen in clownfish, but it can afflict any fish. The fish’s skin will appear to be peeling or sloughing off, oftentimes causing excessive white mucous to form around the affected area(s).
    * Treatment of choice is a 45 minute formalin bath (e.g. Quick Cure), followed by transfer into a new/sterile QT.
    * The fallow (fishless) period for starving Brook out of a Display Tank is 6 weeks.


    Early stages:
    [​IMG][​IMG]
    More advanced stages:
    [​IMG][​IMG][​IMG]
    Additional Information

    Brooklynella hostilis is a ciliate parasite with a direct life cycle: It lives, feeds and reproduces directly on the fish (no encysted stage). However, there always exists the possibility that parasites can drop off into the water column and infect other fish. Having no encysted stage makes this pathogen easier to eliminate, but do not underestimate how fast killing Brook can be. Especially with clownfish.

    Treatment OptionsFormalin is the most effective treatment for Brook, but using Ruby Reef Rally is another good option. Both are best applied via bath treatment, and more information on this can be found in the links below.

    Formalin (45 min bath) https://www.reef2reef.com/threads/formalin.290925/

    Rally (90 min bath) https://www.reef2reef.com/threads/acriflavine.282887/

    Post bath, transfer the fish into a clean/sterile QT to prevent reinfection. It is a wise course of action to dose Chloroquine Phosphate once (60 mg/gal) or metronidazole every 48 hours for 10-14 days thereafter. This is an “insurance policy” to ensure all of the parasites have been eliminated.

    A 5 minute freshwater dip may provide temporary relief if you are unable to locate any of the aforementioned medications right away. Some even claim total eradication of the disease is possible just by performing multiple freshwater dips on the fish. Again, transfer the fish into a new/sterile QT following each freshwater dip to prevent reinfection.
     
    Last edited: Oct 31, 2017

  2. melypr1985

    melypr1985 totally addicted R2R Supporter R2R Excellence Award Article Contributor Expert Contributor Moderator Emeritus

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    i need to see about getting acriflavine for the store. We could use it.
     
  3. Humblefish

    Humblefish Valuable Member R2R Supporter R2R Excellence Award Article Contributor Expert Contributor Moderator Emeritus

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    Very useful for temporary relief of velvet; permanent eradication of brook. A LFS I advise has used it repeatedly to clear clownfish of brook. Acriflavine is not a carcinogen and even has some anti-bacterial properties (two advantages over formalin.) However, it's probably not as "strong" as formalin for treating external protozoa, and I've never tested it on worms (formalin will eradicate flukes, black ich, etc.)
     
  4. revhtree

    revhtree Owner Administrator Staff Member Team R2R R2R Supporter Photo of the Month Award R2R Excellence Award Partner Member 2019 R2R Secret Santa Cyber Monday Sponsor Article Contributor Sizzling Summer Sponsor Build Thread Contributor

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    Great info!
     
  5. ncfishguy

    ncfishguy Well-Known Member

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    Thanks for the support
     
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  6. trahelyk

    trahelyk Active Member

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    How long should fishes with a suspected brook infection remain in QT after symptoms disappear? I have 3 blue damsels who showed signs of brook and have responded to API General Cure, but I worry that such hardy fishes could still be carrying it asymptomatically. How do I know it's safe to move them over to the DT?
     
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  7. Humblefish

    Humblefish Valuable Member R2R Supporter R2R Excellence Award Article Contributor Expert Contributor Moderator Emeritus

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    This is a problem with brook, as I have seen clownfish treated with formalin (bath) and then put right back into the same DT they came out of. They appear asymptomatic for months/years, but then brook comes back following a "stressor event". :confused: If I were in your shoes, I would give all 3 damsels a formalin or acriflavine bath just prior to placing in a DT. Brook is a surface parasite, and 60 minutes in either one of those chemicals should eradicate all traces of it.
     
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  8. trahelyk

    trahelyk Active Member

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    Thanks. As an afterthought... do you think I am correct in diagnosing brook if I saw very fast respiration, decreased appetite, and sloughing white mucous (all over the fish's body, without noticeable distinct spots)?
     
  9. Humblefish

    Humblefish Valuable Member R2R Supporter R2R Excellence Award Article Contributor Expert Contributor Moderator Emeritus

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    The last thing in particular (sloughing white mucous) sounds exactly like brook. Damsels & clownfish are in the same family, so it's logical to conclude damsels are susceptible to brook as well.
     
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  10. LetItReef

    LetItReef Valuable Member

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    @Humblefish This should work to treat Brook? Thanks!

    upload_2016-9-27_10-1-4.png


    upload_2016-9-27_10-4-58.png

    upload_2016-9-27_9-56-24.png

    upload_2016-9-27_9-56-49.png
     
  11. Humblefish

    Humblefish Valuable Member R2R Supporter R2R Excellence Award Article Contributor Expert Contributor Moderator Emeritus

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    @LetItReef Rid Ich Plus is a formalin based medication, so it will treat brook. However, being it only contains 11.52% formaldehyde more than one bath treatment may be required to completely rid a fish of the parasites. I prefer 37% formaldehyde (as found in this product) for a "one & done" bath treatment.

    Formalin-MS is hard to find at most LFS, but it can be purchased online (look here). In a brook emergency, however, I would use the first readily available formalin (or acriflavine) based product.
     
  12. LetItReef

    LetItReef Valuable Member

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    The fastest I can get treatment will be this Thursday. My best bet will be FW dip for 5 minutes, correct? Thanks again!
     
  13. Humblefish

    Humblefish Valuable Member R2R Supporter R2R Excellence Award Article Contributor Expert Contributor Moderator Emeritus

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    Yes, daily 5 min FW dips should buy you some time.
     
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  14. blank

    blank Active Member

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    Hello! I wanted to ask you about the procedure of doing a Acriflavine bath, Ruby reef Rally in particular. How much of it should I put vs cups of tank water for their bath and for how long! (I see 60 minutes and probably 2 baths to be sure?)

    Thanks!
    @Humblefish
     
  15. Humblefish

    Humblefish Valuable Member R2R Supporter R2R Excellence Award Article Contributor Expert Contributor Moderator Emeritus

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    @blank For Ruby Reef Rally, I use roughly 2/3 teaspoon per gallon of bath water to be treated. You can safely overdose acriflavine so exact dosage is not needed.

    One 90 minute bath is normally sufficient for temporary relief of velvet or eradication of brook. However, in some cases fish with the latter may require a second bath.
     
  16. blank

    blank Active Member

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    Sounds good! For 60-70 minutes right?
    Let me just say the plans.
    1) Take saltwater from tank into bucket, add aerator, add about 2/3 teaspoon per gallon.
    2)leave for 60-70 minutes.
    3)Put into qt and feed them some vitamin enriched food (selcon in my case)
    4) wait a week or so and do a bath again.
    5) put into dt?

    This sound good or should I leave my tank fallow a bit longer?
     
  17. blank

    blank Active Member

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    Maybe I'll 1/2 dose into my dt too. It's supposedly reef safe right?
     
  18. Humblefish

    Humblefish Valuable Member R2R Supporter R2R Excellence Award Article Contributor Expert Contributor Moderator Emeritus

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    @blank Your plan sounds fine except for the fallow period. Which disease are you trying to treat? I WOULD NOT use Rally/acriflavine in a reef. Even a FOWLR would be dicey IMHO.
     
  19. blank

    blank Active Member

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    Alright, it seems to be like early signs of brook, I won't dose into the tank .
     
  20. Humblefish

    Humblefish Valuable Member R2R Supporter R2R Excellence Award Article Contributor Expert Contributor Moderator Emeritus

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    D'oh! I forgot you were asking in the Brooklynella thread. :oops:

    Unfortunately, you will need to go fallow for 6 weeks in the DT to starve brook out of there.
     
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