Acriflavine What It Treats – This is an antiseptic that is very useful for rendering “first aid” if a fish has a bacterial infection or to treat an open wound. It can also be used as part of a “three step program” for treating Marine Velvet Disease: 1) 5 minute freshwater dip 2) 90 minute acriflavine bath 3) 30 days of copper or Chloroquine phosphate in a Quarantine Tank (QT) There is some anecdotal evidence that a 90 minute bath using Ruby Reef Rally, followed by transfer into a sterile Quarantine Tank, can clear Brooklynella aka clownfish disease. However, it is unclear whether the acriflavine or formalin Rally contains is the exact reason why. In any case, follow-up treatment using metronidazole in a QT is highly recommended for brook. How To Treat – At present time, only one aquarium product is readily available which contains acriflavine: Ruby Reef Rally. It also contains aminoacridine (another antiseptic) and a small amount of formalin. I personally use Rally as a 90 minute bath, temperature controlling the water and providing plenty of aeration by using an air pump & air-stone. Ensure salinity, pH and temperature of bath & QT water match perfectly. Dosage for Bath is ~ 1 teaspoon of Rally per gallon of bath water. Pros – Effective “pre-treatment” before fish is placed in QT with copper, antibiotics, etc.; milder/safer alternative to using straight formalin (37% formaldehyde). Cons/Side Effects – Turns the water an orange-brownish color, so probably not desirable for Quarantine or Display Tank use. Full range of positive effects and possible side effects are not completely understood at this time.