Coral Coloration Glossary

Very nice list! Just got into reefing this year but my entire career as a scientist is tagging proteins with these fluorescent proteins for live cell imaging. Dronpa is a current progect continuing the work of Dr George H. Patterson the inventor of the photoactivated GFP (NIH) whom we lost just a few weeks ago
Here is an image of a cell with Blue yellow and red fluorescent proteins
 
123.jpg
 
Very nice image! My first impression was that the yellow coloration might be due to color-mixing of GFP and RFP, but apparently not. Was the yellow protein isolated from a zoanthid?
 
I do not isolate the proteins. I only use them to tag proteins of intetest for my research using molecular genetic manipulations. The yellow and cyan fluorescent proteins and their many derivatives were generated by point mutations of the Aquarea Victoria jellyfish GFP. Essentially this image is part of a movie that demonstrates that the vedicle coat protein complex called COP-I moves "forward" in the cell contrary to what is writen in the Cell Biology text books. The proteins are Epsilon COP-YFP, SEC24- mCherry (the red) and the Blue is KDEL-BFP.
 

TOP 10 Trending Threads

WHAT MOST OFTEN MAKES YOU REACH FOR YOUR CAMERA AROUND THE REEF?

  • Coral behavior (feeding, extension, or movement)

    Votes: 0 0.0%
  • New growth or a color change

    Votes: 2 18.2%
  • Unusual fish behavior

    Votes: 0 0.0%
  • The whole tank looking especially good

    Votes: 4 36.4%
  • Something I want to document in case it changes

    Votes: 0 0.0%
  • A funny or unexpected interaction

    Votes: 2 18.2%
  • I usually put the camera/phone down and just watch

    Votes: 1 9.1%
  • Other (Post in the comments)

    Votes: 2 18.2%
Back
Top
Home
Post thread…
Market
What's new