Culturing Phytoplankton Experiment

Reef Pro Corals
OP
Dilan Patel

Dilan Patel

Valuable Member
View Badges
Joined
Dec 25, 2016
Messages
2,132
Reaction score
1,602
Location
Nashville,TN
I can see that some of the bottles are a noticeable amount darker than others. I definitely think that the larger amount of fertilizer made a difference.
Think your right. Ill probably keep the lighter phyto as back ups and use the darker ones rn. im hoping adding the extra food up to 5ml will help me determine the amount of food I should be using.
 

Aqua Man

Active Member
View Badges
Joined
Jan 19, 2020
Messages
305
Reaction score
350
Looking good Dilan. The one does look a tad lighter green.

How often are you splitting?
What salinity is your culture?
Are those 2 liter bottles that you are culturing in?
 
OP
Dilan Patel

Dilan Patel

Valuable Member
View Badges
Joined
Dec 25, 2016
Messages
2,132
Reaction score
1,602
Location
Nashville,TN
this is my second time splitting and today was the 6th day. I did not see any noticeable color change and I was thinking it ran out of food and hit that time period where no growth/very little was happening and was time to split. My salinity for this current batch is 1.019 which is within the range of 1.018-1.020 the card I received said was acceptable. And yes those are 2 L bottles. I kept about 33% of the grown cultures to start the next batch.
 
OP
Dilan Patel

Dilan Patel

Valuable Member
View Badges
Joined
Dec 25, 2016
Messages
2,132
Reaction score
1,602
Location
Nashville,TN
All tools are steralized with White vinegar and rinsed thoroughly with hot water before being used including the culturing vessels..
 
OP
Dilan Patel

Dilan Patel

Valuable Member
View Badges
Joined
Dec 25, 2016
Messages
2,132
Reaction score
1,602
Location
Nashville,TN
I think the left bottle has started to crash? It is significantly lighter than the rest. I found out that 5ml of fertelizer is producing a really nice green so I added 2 more ml's to this bottle to see if it would darken but instead seemed to get lighter. Also is the bigger chunks of phytoplankton a problem?
IMG_1212.jpeg
 
OP
Dilan Patel

Dilan Patel

Valuable Member
View Badges
Joined
Dec 25, 2016
Messages
2,132
Reaction score
1,602
Location
Nashville,TN
no idea why they crashed. 1 had 4ml of food and 1 had 2ml of food. while the other twop had 2ml of food and 5ml of food. So food was not an issue.
 
Top Shelf Aquatics
OP
Dilan Patel

Dilan Patel

Valuable Member
View Badges
Joined
Dec 25, 2016
Messages
2,132
Reaction score
1,602
Location
Nashville,TN
thankfully. I think my problem was the saltwater I used...I used a bag which was rock hard which I just broke up by hand. This basically made some of the mionerals precipitate out and not mix completely clear. This time around I ditched that bag of salt and opened a brand new one not clumped and mixed it until clear.
 
OP
Dilan Patel

Dilan Patel

Valuable Member
View Badges
Joined
Dec 25, 2016
Messages
2,132
Reaction score
1,602
Location
Nashville,TN
All bottles crashed...going back to basics. only going to do 2 cultures filled 1/3 with phytoplankton culture filled the rest with 1.019 SW. All bottles will be sterilized with alcohol instead of vinegar as well as brushed inside. The other componets will be cleaned with alcohol as well. both bottles will have 5ml of food.
 
OP
Dilan Patel

Dilan Patel

Valuable Member
View Badges
Joined
Dec 25, 2016
Messages
2,132
Reaction score
1,602
Location
Nashville,TN
5.0ml per 2 L. and salinity anywhere from 1.020-1.018. With good amount of bubbles. I can usually split within 5 days but let it run for 6-7 because im lazy and don't clean the bottles on time lol.
 

Complaining about the cost of aquarium equipment when you pay large sums of money for coral & fish..

  • is Fair

    Votes: 9 69.2%
  • is Not Fair

    Votes: 4 30.8%
  • Other (please explain)

    Votes: 0 0.0%

Online statistics

Members online
1,692
Guests online
3,522
Total visitors
5,214
Chaos Aquaculture
Top