Dino identification under microscope

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Hi all,

I’m trying to figure out what I’m dealing with in my tank and could use some help with identification.

The tank is about 6 months old. Over the last month while I’ve been getting a dusty brown to greenish coating across the sandbed. It comes back pretty quickly after I disturb it, sometimes within a few hours. It is not really slimy, and I don’t see much of anything on the rocks yet. Some of the heavier areas look a little stringy, but I’m not seeing a lot of bubbles.

Current parameters are roughly:

NO3: 7.7 ppm
PO4: 0.09 ppm
Alk: 8.1 dKH
Salinity: 35 ppt

I was really careful with nutrients and even dosed nitrate and phosphate when they were close to 0. Not sure why I still got dinos. I did clean the sandbed quite often during water change. Maybe that mess up the biome.

I picked up a microscope and sampled the film directly from the sand. I’m seeing quite a few golden brown oval cells that are actively moving. I attached a video of them under the microscope.

Does this look like Amphidinium, Prorocentrum, or something else?


Also interested in what has worked best for people dealing with this type. I already run UV, although I understand it may not help much if this is large cell Amphidinium. I’m curious about silicate dosing, encouraging diatoms, reducing sandbed cleaning, or anything else that has worked long term.

Thanks for the help!

 
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20260910_214214_ECEDA191-24A0-45B1-BF6A-B1791671C1BA.png

Here is the sandbed under white light
 
Yes this is Large Cell/Small cell. The small cell ones are fast moving. They cover rock and sand and burrow when lights go out. Bacteria dosing using Mb7 or MicrobLift special blend. You need to outcompete them. Increase your microbiome this could be done by adding Aflifesource or live sand/rock. Silicate dosing is another method. This is how I beat large/small cell. The aftermath was large amounts of green hair Algea
 
Yes this is Large Cell/Small cell. The small cell ones are fast moving. They cover rock and sand and burrow when lights go out. Bacteria dosing using Mb7 or MicrobLift special blend. You need to outcompete them. Increase your microbiome this could be done by adding Aflifesource or live sand/rock. Silicate dosing is another method. This is how I beat large/small cell. The aftermath was large amounts of green hair Algea
Thanks for the reply. Does this type of dino cause immediate harm that need to be dealt with quickly? Is silicate dosing a faster/more aggressive approach?
 
Thanks for the reply. Does this type of dino cause immediate harm that need to be dealt with quickly? Is silicate dosing a faster/more aggressive approach?
It’s one of the least toxic Dino’s. It won’t harm fish at least from my experience. But can harm sensitive corals like sps. If inverts eat it can kill them. They usually won’t touch it though. The problem is when Dino’s take over not much other Algea have the realestate to grown and as a result inverts starve. Throw in some Algea pellets or seaweed during this phase.

Another thing make sure your Nitrate and Phosphate isn’t bottomed out if it is raise them. It could take months to beat Dino’s

Silicate approach is aggressive and your take will look horrible. I was siphoning the Dino strands into a 1micron sock and returning the water back into the tank
 
When you get them beat back. Hit them with some real ocean live rock and some sand. This is what finished them off for me. I think that diversity helps get things back in balance.
 
Do you have livestock? Have you considered adding a sand sifting fish? While it is unsightly, some of this is usually mediated by normal livestock activity, and unless it's big clumps or its encroaching on corals, it's not likely to be a big negative impact on the livestock.

May not directly effect the dinos, but your phosphate is low relative to your nitrate - both are reasonable levels, but having too much nitrate proportionally can throw things out of whack a bit. I think it's easier to maintain a slightly higher phosphate and use about that for your nitrate level.

Did you start with live rock? 6 months should be plenty of seasoning time with live rock in a reasonable quantity and livestock added later, but if you started with dry rock and held off on adding corals for months, it could be still that you're working through the early stage uglies.
 
I agree with the "Amphidinium" identification (see post #3, page 1). Regarding the toxins I'm careful with giving a verdict because toxin production of many dinoflagellates appears to be strain and environment-dependent and we don't even know the species.

You can certainly try adding bottled bacteria products, but they can just as well provide another food source for the dinoflagellates you are dealing with. This post (page 1, post #4) explains why many products won't necessarily provide much living bacteria at all and judging from the bacteria actually contained in those bottles, the "diversity" aspect is negligible with 3 - 10 species in tanks that house hundreds of species.

In my opinion manual removal and water changes are definitely beneficial. People are always afraid of the introduced trace elements that will support dinoflagellate growth but without those trace elements competing organisms won't have a chance establishing either.

If that doesn't work silicate dosing is certainly another option and you can actually see organisms competing over the same space (and therefore light) with those dinos. Some dinos still prey on diatoms though, so I think you need to get the diatom numbers up by a lot to actually have a beneficial effect. It will leave the substrate discolored for quite a while and the glass will need to be cleaned more frequently, but diatoms don't really hurt your system.
 
Do you have livestock? Have you considered adding a sand sifting fish? While it is unsightly, some of this is usually mediated by normal livestock activity, and unless it's big clumps or its encroaching on corals, it's not likely to be a big negative impact on the livestock.

May not directly effect the dinos, but your phosphate is low relative to your nitrate - both are reasonable levels, but having too much nitrate proportionally can throw things out of whack a bit. I think it's easier to maintain a slightly higher phosphate and use about that for your nitrate level.

Did you start with live rock? 6 months should be plenty of seasoning time with live rock in a reasonable quantity and livestock added later, but if you started with dry rock and held off on adding corals for months, it could be still that you're working through the early stage uglies.
I added about 5 lbs of live rock in the sump at the beginning and have been adding corals constantly. One thing I suspect is overclean the sand. I siphon the detritus quite aggressively during the weekly water change. Maybe that threw off the bacteria balance. What's the best way to raise only phosphate? I've been draining out the froze food liquid, maybe pour those into the tank as well?
 
I agree with the "Amphidinium" identification (see post #3, page 1). Regarding the toxins I'm careful with giving a verdict because toxin production of many dinoflagellates appears to be strain and environment-dependent and we don't even know the species.

You can certainly try adding bottled bacteria products, but they can just as well provide another food source for the dinoflagellates you are dealing with. This post (page 1, post #4) explains why many products won't necessarily provide much living bacteria at all and judging from the bacteria actually contained in those bottles, the "diversity" aspect is negligible with 3 - 10 species in tanks that house hundreds of species.

In my opinion manual removal and water changes are definitely beneficial. People are always afraid of the introduced trace elements that will support dinoflagellate growth but without those trace elements competing organisms won't have a chance establishing either.

If that doesn't work silicate dosing is certainly another option and you can actually see organisms competing over the same space (and therefore light) with those dinos. Some dinos still prey on diatoms though, so I think you need to get the diatom numbers up by a lot to actually have a beneficial effect. It will leave the substrate discolored for quite a while and the glass will need to be cleaned more frequently, but diatoms don't really hurt your system.
Looks like AF life source is the way to go if I want to try the bateria product route? Do you know how long it would take to see an improvement? Right now it's still limited to the sand but I don't want it to grow out of control before I switch to silicate
 
I agree with the "Amphidinium" identification (see post #3, page 1). Regarding the toxins I'm careful with giving a verdict because toxin production of many dinoflagellates appears to be strain and environment-dependent and we don't even know the species.

You can certainly try adding bottled bacteria products, but they can just as well provide another food source for the dinoflagellates you are dealing with. This post (page 1, post #4) explains why many products won't necessarily provide much living bacteria at all and judging from the bacteria actually contained in those bottles, the "diversity" aspect is negligible with 3 - 10 species in tanks that house hundreds of species.

In my opinion manual removal and water changes are definitely beneficial. People are always afraid of the introduced trace elements that will support dinoflagellate growth but without those trace elements competing organisms won't have a chance establishing either.

If that doesn't work silicate dosing is certainly another option and you can actually see organisms competing over the same space (and therefore light) with those dinos. Some dinos still prey on diatoms though, so I think you need to get the diatom numbers up by a lot to actually have a beneficial effect. It will leave the substrate discolored for quite a while and the glass will need to be cleaned more frequently, but diatoms don't really hurt your system.
Looks like AF life source is the way to go if I want to try the bateria product route? Do you know how long it would take to see an improvement? Right now it's still limited to the sand but I don't want it to grow out of control before I switch to silicate
I would use Tropic Marin Nitribiotic because those bacteria actually survive being bottled.
AF lifesource has a large "diversity" but the actual number of viable cells is probably much lower.

I will have to conduct a few tests before giving my actual judgement on AF life source though. From a diversity standpoint I really wish it has viable cells.
 
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5 pounds and added corals should give you enough of a microbiome innoculation to be fine within 6 months, imo, so while things still may be settling, I don't think you're like missing critical components or anything.

People say frozen foods are good sources of phosphate (I think it's just that they are less concentrated sources of nitrate), and I think the fines in the liquid are beneficial for small mouths and plankton feeders, so I'd just thaw it in tankwater and feed everything (what I do.) It may also be possible to just feed more, depending on tank size, bioload, etc. - this hobby has a long history of underfeeding in favor of algae/parameter management and it's not always to the benefit of some of the livestock.

But while I would make sure I was feeding enough and monitor things - is what you see really a problem? If it's encroaching on corals and killing them, yeah you are right to want to remove it, but if it's just sort of looking ugly... I think less intervention can sometimes lead to less dramatic shifts to correct for changes and ultimately a quicker path to long term success. Some amount of cosmetic maintenance is fine, but I'm not sure big course correction is necessary here unless things are being harmed.
 
5 pounds and added corals should give you enough of a microbiome innoculation to be fine within 6 months, imo, so while things still may be settling, I don't think you're like missing critical components or anything.

People say frozen foods are good sources of phosphate (I think it's just that they are less concentrated sources of nitrate), and I think the fines in the liquid are beneficial for small mouths and plankton feeders, so I'd just thaw it in tankwater and feed everything (what I do.) It may also be possible to just feed more, depending on tank size, bioload, etc. - this hobby has a long history of underfeeding in favor of algae/parameter management and it's not always to the benefit of some of the livestock.

But while I would make sure I was feeding enough and monitor things - is what you see really a problem? If it's encroaching on corals and killing them, yeah you are right to want to remove it, but if it's just sort of looking ugly... I think less intervention can sometimes lead to less dramatic shifts to correct for changes and ultimately a quicker path to long term success. Some amount of cosmetic maintenance is fine, but I'm not sure big course correction is necessary here unless things are being harmed.
All living beings I'm aware of have N:P ratios above 1 so there is no natural food you will find that adds more phosphate than nitrate. It's just immediately measurable in the thawed water (see https://www.reef2reef.com/threads/f...affect-phosphate-levels-in-aquariums.1129634/)

Adding food also increases organics so if you want to control nitrogen or phosphorus availability I always recommend using DIY solutions rather than overfeeding or you will just pollute the tank with all sorts of stuff while never being able to actually control how C, N, and P will ultimately behave in the tank.
 
5 pounds and added corals should give you enough of a microbiome innoculation to be fine within 6 months, imo, so while things still may be settling, I don't think you're like missing critical components or anything.

People say frozen foods are good sources of phosphate (I think it's just that they are less concentrated sources of nitrate), and I think the fines in the liquid are beneficial for small mouths and plankton feeders, so I'd just thaw it in tankwater and feed everything (what I do.) It may also be possible to just feed more, depending on tank size, bioload, etc. - this hobby has a long history of underfeeding in favor of algae/parameter management and it's not always to the benefit of some of the livestock.

But while I would make sure I was feeding enough and monitor things - is what you see really a problem? If it's encroaching on corals and killing them, yeah you are right to want to remove it, but if it's just sort of looking ugly... I think less intervention can sometimes lead to less dramatic shifts to correct for changes and ultimately a quicker path to long term success. Some amount of cosmetic maintenance is fine, but I'm not sure big course correction is necessary here unless things are being harmed.
Honestly it's not causing any issues right now, they are limited to the sand. I'm more concerned because of what I read about Dino. I don't want to only start treatment when it gets out of control. Would dino's improve on their own?
 
Honestly it's not causing any issues right now, they are limited to the sand. I'm more concerned because of what I read about Dino. I don't want to only start treatment when it gets out of control. Would dino's improve on their own?
Dinos can stay limited to the substrate basically "forever." For the situation to improve, something has to change, but as long as they aren't overgrowing anything and aren't producing significant amounts of toxins the tank can't handle, they aren't a problem.
 
Dinos can stay limited to the substrate basically "forever." For the situation to improve, something has to change, but as long as they aren't overgrowing anything and aren't producing significant amounts of toxins the tank can't handle, they aren't a problem.
Exact, but lets remember that dino is in normal amount not something bad but a natural ingredient in the ecosystem. The zooxanthelle is a dinoflagellate for instance 🙂
 
Honestly it's not causing any issues right now, they are limited to the sand. I'm more concerned because of what I read about Dino. I don't want to only start treatment when it gets out of control. Would dino's improve on their own?
Dino may sometimes go away by itself yes. But not always and can explode very fast. So my message is, do not wait too long. "My" method with uvc and blackout doesnt harm anything else so you should have a low threshold to do that.

When dino is gone you will see an increase in po4 and no3. Po4 I wouldn't concern, will go down by itself, but your nitrate of already 33 ppm is a problem. We nay have different opinions here but AMO its not good for the corals, especially not with other stressors (there is scientific studies supporting this).

But I would not use carbon. That is uncontrolled and will also take down po4 and thats not what you want(and can actuallyalso feed dino!) . You need a selective reduction =denitrification.
You can try those anaerob block from maxspect. They work. (The black ones)
Maybe buy 3. It takes 1-2 months and then they kick in.
I have 2 of them. I had 15ppm in nitrate before, now 3 ppm.

Jonas
 
I’m trying to figure out what I’m dealing with in my tank and could use some help with identification.
I recommend posting here for the most wholistic treatment that was put together by and excellent team of coconspirators here on R2R – not by an single person. Almost all of your questions (even ones you don't have yet) are answered in the first post:
https://www.reef2reef.com/threads/dinoflagellates-–-are-you-tired-of-battling-altogether.293318/

If you haven't already read page one as well as the linked journal articles there, do that as well. (reading the whole thread is not necessary)

The tank is about 6 months old.
Has it already gone through The Uglies? How was it and did you try to do anything about it? (What did you do?)

Thanks for the reply. Does this type of dino cause immediate harm that need to be dealt with quickly? Is silicate dosing a faster/more aggressive approach?
The question is how are your pods and cleanup crew? If they are OK then no toxins.

No toxins and you can worry about this more like a green algae bloom. Your tank isn't going to end up a giant dead zone....you just have a lot of freindly dino's. Maybe more than you'd like, but it's a stretch to call it a problem compared to a normal dino bloom where nothing by cyano and dino's survive.

If you do have signs of toxins, then you should be running a 1/4 portion of activated carbon changed out weekly instead of monthly.

Current parameters are roughly:

NO3: 7.7 ppm
PO4: 0.09 ppm
These are not parameters of a typical toxic dino bloom, so again how are your tanks signs of toxicity?

I picked up a microscope and sampled the film directly from the sand. I’m seeing quite a few golden brown oval cells that are actively moving. I attached a video of them under the microscope.
I'm curious how representative this slide is if you take a few more samples.

Do you see nothing but dino's or do some slides show more lifeforms? I would hope to find a variety of things, almost no matter what that variety is.

Also interested in what has worked best for people dealing with this type. I already run UV, although I understand it may not help much if this is large cell Amphidinium. I’m curious about silicate dosing, encouraging diatoms, reducing sandbed cleaning, or anything else that has worked long term.
There is not a cut and dried method for these. Lucky you! 🫤

Silicate dosing, like most other attempted treatments, seems to have proved very hit or miss.....which is not a sign of a good solution. 50/50 odds are similar odds to random guessing at a solution or doing nothing at all. H2O2, blackouts, bleach, Fill In The Blank products, etc all seem to get about 50/50 odds when you look at enough examples.

If it's really true that they ALL burrow into the substrate at night, I don't see why *someone* hasn't had some success by siphoning out the whole sandbed at night. Maybe nobody has tried at night?

Sand bed replacement typically does practically nothing though.....so I'm not so sure about the "all go into the sandbed" theory.

I think we're missing something....perhaps only adults have this behavior and the babies (swarmers) are still waterborn....I'm not sure though.

Another thing I don't think I've seen someone try is taking good nutrient levels like yours and purposefully lowering them to threshold levels.....nutrient changes like this are one of the common signals for a bloom to change form and to end. But there are other signals, such as salinity and temperature that are often "mixed in" and that complicates things.
In my opinion manual removal and water changes are definitely beneficial. People are always afraid of the introduced trace elements that will support dinoflagellate growth but without those trace elements competing organisms won't have a chance establishing either.
Overall agreed. The trace element theory is very old (and bunk) though. By spiking water changes with spec levels of N and P we prevented those "relapse" symptoms you're referring to.....definitely seemed related to P (and N) being bottomed out more than other factors.

I added about 5 lbs of live rock in the sump at the beginning and have been adding corals constantly.
In the sump is not great placement if you're expecting that rock to seed the display tank somehow. Displace some dead display rock withe the live rock and put the dead in the sump or better yet put it up for sale.

One thing I suspect is overclean the sand. I siphon the detritus quite aggressively during the weekly water change. Maybe that threw off the bacteria balance.
No. If anything removing dino's is what you were doing...and it should be good to do that even more.

Consider siphoning into a 25 micron (or smaller if possible) filter sock or equivalent and then adding the water back to the tank. This will allow you do do almost unlimited siphoning for cleaning purposes.

What's the best way to raise only phosphate? I've been draining out the froze food liquid, maybe pour those into the tank as well?
LOL, no...can't believe all the things they have people worrying about these days. Sheesh!

Use a phosphate product like Seachem Flourish Phosphate. In general I'd target ≥ 0.10 ppm for phosphates....though you're already close to that, aren't you?

Looks like AF life source is the way to go if I want to try the bateria product route? Do you know how long it would take to see an improvement? Right now it's still limited to the sand but I don't want it to grow out of control before I switch to silicate
If you have plenty of funds and time to put into experiments, then maybe. IMO there is no "competition" between bacteria and dino's where "bacteria win". That's a narrative cut from whole cloth that folks apply to all kinds of pests to no avail.

Honestly it's not causing any issues right now, they are limited to the sand. I'm more concerned because of what I read about Dino. I don't want to only start treatment when it gets out of control. Would dino's improve on their own?
Don't freak yourself out. Read the thread I posted at least enough to see which groups of dino's DO CAUSE HAVOK...so you can realize you don't have them and stop freaking yourself out. :)

"My" method with uvc and blackout doesnt harm anything else so you should have a low threshold to do that.
I will give you a parital retraction of the bunk label I mentioned in relation to blackouts elsewhere.....in a case like this (no stress, no damage, no indication of a real problem) a blackout should be fine as nothing is already in a state of stress or decline. Should be pretty harmless to try, I agree.
 
Dinos can stay limited to the substrate basically "forever." For the situation to improve, something has to change, but as long as they aren't overgrowing anything and aren't producing significant amounts of toxins the tank can't handle, they aren't a problem.
Exact, but lets remember that dino is in normal amount not something bad but a natural ingredient in the ecosystem. The zooxanthelle is a dinoflagellate for instance 🙂
I never said something contradicting this statement 🤨
 

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