Dinoflagellate Problem? Could Ulva lactuca be the Answer?

bluewill

Active Member
View Badges
Joined
Jan 30, 2018
Messages
100
Reaction score
40
Location
Fort Lauderdale, FL
Rating - 0%
0   0   0
following ,
and researching a possible why to this phenomena

The below excerpts were taken from various research articles, I read last night . My pre conclusion is that their is quantifiable evidence as to why this may have worked in "itsanames" system, and would definately induce future discussion and experiments
FYI their were many more references apllicable

"The ethyl acetate extracts of U. lactuca, U. fasciata and U. reticulata showed the presence of phytochemicals, terpenoids, tannins and phenolic compounds strongly than the other extracts. The finding suggested that ethyl acetate extracts of U. lactuca, U. fasciata and U. reticulata exhibited an antifungal substance for the treatment of fungal infections. "

"
Sargassum, Gracilaria and Ulva Exhibit Positive Antimicrobial Activity against Human Pathogens"

"This work shows the capabilities of living seaweed, Ulva lactuca, to remove As, Cd, Pb, Cu, Cr, Hg, Mn and Ni from contaminated waters. Experiments were performed with three algal doses (1.5, 3.0 and 6.0 g L-1, FW), two ionic strengths (salinity 15 and 35), and trace element concentrations corresponding to the maximum allowed values in wastewaters. The highest removals were obtained with the algal dose of 6 g L-1, with efficiencies varying between 48% for As and 98% for Hg, after 24 to 72 h. Salinity showed no effect on the removal efficiency. Overall, Elovich model was the best in describing the kinetics of the process, except for Hg, where pseudo-second-order model performed better. The use of extractions with EDTA (0.001, 0.01 to 0.1 mol L-1) has clarified that most of the Hg (≈98%) and Cr (≈80%) crossed the macroalgae walls, while Ni, Cd and As were retained at the surface (between 60 and 80%). These results support the hypothesis that
---------------------------------------------
"Submerged aquatic macrophytes are important in shallow aquatic ecosystems because they stabilize the macrophyte-dominated state by increasing water transparency in various ways. One of these is the excretion of allelopathic substances inhibitory to phytoplankton,
----------------------------------------
"Many species of ulvoid green algae produce allelochemicals whose effects include reducing densities of barnacles in tidepools (Magre, 1974); causing mortality in crab larvae (Johnson and Welsh, 1985), oyster larvae ( Nelson et al., 2003a;Nelson and Gregg, 2013), and juvenile abalone C15 11/15/2017 17:9:28 Page 542 ( Wang et al., 2011); inhibiting the growth of planktonic microalgae (Jin and Dong, 2003;Wang et al., 2009;Tang and Gobler, 2011) and benthic macroalgae (Nelson et al., 2003a); and reducing fouling by epiphytic bacteria, algae, and invertebrates ( Egan et al., 2000;Nelson et al., 2003b;Harder et al., 2004;Hellio et al., 2004). ...
-----------------------------------------
"This study demonstrated that the green macroalga, U. lactuca,is
capable of restricting the growth of seven species of phytoplankton
that form HABs across the globe. The occurrence of these impacts
in the presence of high nutrient concentrations, undetectable
bacterial concentrations, and normal pH levels implicates allelo-
chemicals as a causative mechanism in the growth reduction of
HABs. The ability of aqueous extracts of macroalgae or live thalli of
macroalgae connected to HABs through a 5-
m
m mesh to restrict
HAB growth demonstrated that the dry powder of U. lactuca
contains water-soluble and heat-stable allelochemical(s) respon-
sible for inhibiting the growth or lysis of the cells of microalgae and
that direct contact is not required for the effects of U. lactuca on
these Harmful Algea Blooms species to occur. Collectively, these results provide new
insight into the ecological role of macroalgae such as U. lactuca in
the occurrence of HABs."
----------------------------------------------
"Based on prior research conducted with species of Ulva from
Asia, some of the allelochemicals produced by this alga may
include polyunsaturated fatty acids (Alamsjah et al., 2005, 2008).
Dithiolane and trithiane compounds isolated and identified from
Characean species were also observed to cause allelopathic effects
on epiphytic diatoms and other phytoplankton (Wium-Andersen
et al., 1982). Our results demonstrated that the dried or heated
extracts of U. lactuca were equally or more potent than the
equivalent thalli, an observation consistent with the hypothesis
that polyunsaturated fatty acids or organosulfur compounds are
active allelopathic agents. The enhanced potency of the extracts
also suggests that allelochemicals in cells were more concentrated
than the portion released into the environment."

read full text

https://www.researchgate.net/public...n_Harmful_Algal_Bloom_Species_via_Allelopathy
--------------------------------------
"The results of experiments using the U. lactuca extract after
adjustment of pH to 8.5 showed similar cell lysis effects in the four
target species, A. anophagefferens,P. minimum,K. veneficum, and C.
marina during 48 h incubations and no live cells were observed
under an inverted microscope for all test species at 48 h,"
-------------------------------------
Allelopathic effects of several concentrations of fresh tissue, dry powder and dry tissue of three bloom-forming green macroalgae Ulva pertusa, Ulva linza and Enteromopha intestinalis on the red tide microalga Heterosigma akashiwo were evaluated in microcosms systems. The effects of macroalgae culture medium filtrate were investigated on H. akashiwo using initial or semi-continuous filtrate addition.the growth of H. akashiwo was significantly inhibited under semi-continuous filtrate addition, which suggests that continuous release of small quantities of rapidly degradable allelochemicals from the fresh tissue of both macroalgae was effective in inhibiting the growth of H. akashiwo.

read this paper

https://www.researchgate.net/public..._Pharmacology_and_Harmful_Algae_Bloom_Control
-----------------------------------
"The isolation of natural compounds from many aquatic and terrestrial plants and seaweeds has become an alternative approach for controlling harmful algae in aquatic systems. Seaweeds have received attention from scientists because of their bioactive compounds with antibacterial, antifungal, anti-microalgae, and antioxidant properties. The undesirable effects of cyanobacteria proliferations and potential control methods are here reviewed, focusing on the use of potent bioactive compounds, isolated from seaweeds, against microalgae and cyanobacteria growth.
(PDF) Seaweed Bioactive Compounds against Pathogens and Microalgae: Potential Uses on Pharmacology and Harmful Algae Bloom Control. Available from:


not related but interesting
"Clay dispersal is one of only a few mitigation methods for harmful algal blooms (HABs) ever applied in the field; however, low flocculation efficiency has always been the most significant drawback associated with natural unmodified clays. This review discusses key factors affecting the flocculation efficiency, based on results obtained in studies of the mechanisms underlying interactions between clay particles and HAB organisms. It further elaborates clay surface modification theory and methods for improving removal efficiency of HAB cells, followed by descriptions of various modified clays successfully prepared with removal efficiencies of HAB cells that are up to hundreds of times greater than natural clays and have lower dosing requirements of 4–10 t/km². Presently, modified clays are the most widely used method for the mitigation of HAB in the field in China. This review also evaluates potential ecological effects of modified clay disposal on water quality, typical aquatic organisms, benthic environments, and ecosystems. Both laboratory and field results have demonstrated that modified clays markedly can actually improve water quality after treatment and pose no negative effects on aquatic ecosystems."
 

bluewill

Active Member
View Badges
Joined
Jan 30, 2018
Messages
100
Reaction score
40
Location
Fort Lauderdale, FL
Rating - 0%
0   0   0
This article here https://www.mdpi.com/1660-3397/16/2/55/htm
was very informative
conclusion below


4. Conclusions
Enhanced growth of aquatic vegetation or phytoplankton and algal blooms disrupts normal functioning of aquatic ecosystems all over the world. When toxic microalgae and cyanobacteria are involved in these eutrophication consequences, a variety of ecological, economical, and sanitary health problems could arise. Most of the recent studies on the control of the HABs have focused on the use of chemical, physical, and biological treatment agents but these processes show serious environmental consequences. Among the biological agents, a variety of extracts from aquatic and terrestrial plants, which contain many bioactive compounds, with a wide range of applications and biocides activities have been experimented.
With respect to bioactive compounds extracted from seaweeds, most of them have been applied for their biocidal (anti-fungi, anti-bacteria) and pharmaceutical activities. However, very few reports have focused on their algicide and anti-cyanobacterial activities. In order to explore macroalgae as an alternative and an available natural source of bioactive compounds, we recommend that works could be oriented on the research of new natural products extracted from seaweeds. Seaweed extracts show interesting potential against many harmful microalgae and cyanobacteria species but not much is yet known, namely the structure and mechanisms of action of the effective substances. These substances should be tested for their biocide activities against micro-algae growth in general and particularly against cyanobacteria growth. Research on novel biomolecules is needed in order to better control the phytoplankton excessive growth in a sustainable way, and to maintain the ecological equilibrium and the stability of the aquatic ecosystems.
 

Mortie31

Valuable Member
View Badges
Joined
Apr 5, 2016
Messages
1,789
Reaction score
2,963
Location
Uttoxeter. England
Rating - 0%
0   0   0
Ulva is really available in the UK to reefers a few sell it online, when I set my new fuge up I will get hold of some. What are all of your thoughts on it’s potential effect or lack of on SPS corals?
 

Mortie31

Valuable Member
View Badges
Joined
Apr 5, 2016
Messages
1,789
Reaction score
2,963
Location
Uttoxeter. England
Rating - 0%
0   0   0
Ulva is really available in the UK to reefers a few sell it online, when I set my new fuge up I will get hold of some. What are all of your thoughts on it’s potential effect or lack of on SPS corals?
Sorry all it’s a different strain that’s available it’s Ulva Intestinalis
 
OP
OP
Scrubber_steve

Scrubber_steve

2500 Club Member
View Badges
Joined
Feb 19, 2018
Messages
3,224
Reaction score
4,801
Location
down under
Rating - 0%
0   0   0
I have an outdoor pond with koi/goldfish. It gets full sun for a few hours each day, & so algae can be a problem.
The water is always crystal clear because I use UV to kill whatever micro algae goes through it, but macro (very fine hair algae) grows on the surfaces.
I cleaned it out completely the other week, & got rid of 95% of the macro. But it will grow back, & I like a little bit in any case, but!!

I might do some testing using the species of ulva I grow on my reef tank scrubber screen to see if it has any affect on this 'freshwater' macro?
Ill sun dry it out & powder it & add it to the pond, see what happens?
 
OP
OP
Scrubber_steve

Scrubber_steve

2500 Club Member
View Badges
Joined
Feb 19, 2018
Messages
3,224
Reaction score
4,801
Location
down under
Rating - 0%
0   0   0
What are all of your thoughts on it’s potential effect or lack of on SPS corals?
I grow a different species of ulva in my scrubber, so cannot vouch for lettuce ulva (U lactuca) specifically. Mine might be intestinalis going by some photos (https://c1.staticflickr.com/2/1680/26489550950_84eb616f53_b.jpg), then again it may not be?
I have no algae other than coralline in my display; the green & brown hair algae & cyano that I did have disappeared after the algae scrubber screen matured.

upload_2018-12-24_9-51-59.png
 

bluewill

Active Member
View Badges
Joined
Jan 30, 2018
Messages
100
Reaction score
40
Location
Fort Lauderdale, FL
Rating - 0%
0   0   0
Researched this all day
theirs actually a lot more research information than I thought would be available .

this was probably the most detailed research specifically on Ulva Lactuca that I read :

https://www.researchgate.net/public...n_Harmful_Algal_Bloom_Species_via_Allelopathy

scroll down to Authors copy to read

I would'nt assume that you guys would start buying these products and dumping them in your tanks, or am I wrong ?
Im not saying that we shouldn't check this out but hopefully if you do , you will document thoroughly

Obviously the ideal initial plan for experimentation would be to buy some live samples of Ulva Lactuca for addition to refugum , or tank,. there are lots of different species of Ulva , and Im sure the have different characteristics .

their are extracts , but not sure whats in them
their are powders :
this one you can see ingredients and looks pure
https://www.amazon.com/100Mesh-Lact...99050&sr=8-2&keywords=sea+lettuce+powder&th=1

not sure about these but sent some info requests
https://www.amazon.com/Sea-Lettuce-...1545609438&sr=8-5&keywords=sea+lettuce+powder
https://www.ebay.com/itm/Organic-Se...h=item3b24ee7928:g:oJEAAOSwoJNa3PWL:rk:6:pf:0

flakes:
https://www.ebay.com/itm/Organic-Se...=item3d7caaf305:g:eMIAAOSwwNRa3PUJ:rk:11:pf:0




(
 
OP
OP
Scrubber_steve

Scrubber_steve

2500 Club Member
View Badges
Joined
Feb 19, 2018
Messages
3,224
Reaction score
4,801
Location
down under
Rating - 0%
0   0   0

ItsAName

Active Member
View Badges
Joined
Oct 21, 2016
Messages
404
Reaction score
199
Rating - 0%
0   0   0
Did you just add it to a refugium or did you dry it/grind it and add it to the tank?

I added it to the refugium. It grows like a weed, I would say doubles its volume every 7-14 days. I never heard about drying it and grinding it.
 

ItsAName

Active Member
View Badges
Joined
Oct 21, 2016
Messages
404
Reaction score
199
Rating - 0%
0   0   0
L lactuca would have outcompeted the diatoms for nutrients & some necessary elements, possibly even assimilated silica? Did alleochemicals play a part?

I don't know what you mean by Alleochemicals? When I originally posted about it, as you said, the consensus was that it outcompeted the diatoms for the silica.
 

Cory

More than 25 years reefing
View Badges
Joined
Oct 30, 2014
Messages
6,882
Reaction score
3,105
Location
Canada
Rating - 0%
0   0   0
So its been about 48 hours and the dinos havent receeded from new life spectrum algae max. Its second ingriendient is ulva. Will have to try live ulva later. Since the paper said 48 hours had a mass mortality in dinos i havent seen it. Unless they are dead just stringy still. Not sure.
 

Cory

More than 25 years reefing
View Badges
Joined
Oct 30, 2014
Messages
6,882
Reaction score
3,105
Location
Canada
Rating - 0%
0   0   0
I don't know what you mean by Alleochemicals? When I originally posted about it, as you said, the consensus was that it outcompeted the diatoms for the silica.

Allochemicals are chems that some things release that kill other things. Ulva has allochemicals that kill the listed dinos according to the paper.
 

TOP 10 Trending Threads

WHAT AMOUNT OF LIVE ROCK AND SAND SHOULD BE PRIORITIZED FOR OPTIMAL BIODIVERSITY/FILTRATION?

  • 100% live rock + bagged sand

    Votes: 38 27.1%
  • 100% dry rock + 100% live sand

    Votes: 47 33.6%
  • 50/50 live/dry rock, 50/50 live/bagged sand

    Votes: 31 22.1%
  • 75% live rock, 25% live sand

    Votes: 14 10.0%
  • 25% live rock, 75% live sand

    Votes: 10 7.1%
Back
Top