it is fun to dissect which conditions would alter the filtration bacteria in our tanks measurably
in this case, the holding container for the bac
so many things are claimed to kill or destabilize bacteria-big water changes are one
removing the sand
moving the rocks
having the rocks in the air
the actions that truly kill bacteria in our tanks to the point filtration (ammonia oxidation) becomes measurably altered are highly limited but this could be a case of one set of variables imo. many times its not loss of bac registering an ammonia event, its a dieoff of some sort that overcomes bac otherwise doing just fine having been through the same event that killed a macro animal
would be curious to see if any of that bac survived inside the container after thawing. I suspect some will, and many will have died. temp extremes are on the scale of antibiotic measures for isolated cultures so I wouldn't be surprised either way
TW want to know something hilarious, I need yours or Jasons scoping skills for a project
I have a He man snake man from 1984 (where you press on his head and he squirts water) that is in a toy case and still filled from my last 4th grade venture with it. shake it, and his chest cavity is filled with El Paso tap water from the east side in 1984 lol
33 years locked in PBA infested dyed plastic, in the dark, with only the original fill contaminants in tow. Would it self sterilize? id be willing to mail it out, we'd have to devise a nice aseptic extraction technique to get down inside the neck and extract the sample, but what a neat article that would make. people could state their predictions ahead of time before the results. I predict sterile.