Is prime the same as conditioner? I have been using conditioner for the new freshwater.
Like someone else said above, get some Fritz Turbo 9000. It will be refrigerated. You could also use the unrefrigerated 900 or Dr Tims. You need to start building that bacteria population ASAP. Your ammonia should read 0 ppm. Anything higher is harming your inhabitants. Prime will rid the ammonia, but it is only a bandage for the bigger issue of needing more nitrifying bacteria.
Edit:
Your nitrates look high because you have nitrite in your tank. That gives you a false high reading on your nitrates. Both ammonia and nitrite should read 0 ppm. I wouldn't bother reading nitrate until both ammonia and nitrite are 0 ppm.