Our tank's biology up close

Discussion in 'Reef Chemistry by Randy Holmes-Farley' started by jason2459, Mar 15, 2016.

  1. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Nothing at all to do with the above strand that the bacteria are attached to but very cool

    Bacteria nano wires
    Http://phys.org/news/2014-08-bacterial-nanowires-thought.amp

    "Shewanella bacteria roughly 1/50th the width of a human hair reach out nanowires to breathe when deprived of oxygen. These types of bacteria are typically found underground or in lake or ocean sediments, where oxygen is scarce. Credit: PNAS, DOI: 10.1073/pnas.1410551111"

    http://m.pnas.org/content/111/35/12883


    And more cool research on bacteria immune system that just got published yesterday
    http://m.phys.org/news/2016-11-bacteria-ramp-immune-response-viral.html
     
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  2. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    I did get a closer look tonight at Vibrant. And I keep wondering if I've done something to cause those stands to appear. I'm starting to think not. Especially since I haven't seen them in any other samples I've ever done with bacteria in a bottle, in tank's samples, or many other samples I've done with my son from around the house, body, and yard.

    Uploading pics and vids now. To make sure it wasn't how I prepared (cleaned/sterilized) the slides I looked at a sample completely unprepared. Thinking maybe it's the cover slip somehow I viewed another sample with out the coverslip and in a well slide. Then used a little stain and rinsing. Etc.

    Found strands in every instance. In some cases I found lots as if I smeared the sample and stain which I didn't.

    Then the last sample I looked at I found the mother of all lines...

    No idea what it is. It doesn't stain with violet or iodine or safranin. But that last "line" was very interesting and had a few even thinner stands starting off it.


    Edit :
    it's not entirely dormant. There was some active movement.
     
    Last edited: Nov 21, 2016
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  3. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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  4. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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  5. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Another sample with out cleaning the slide thinking maybe I'm doing something in the cleaning process causing streaks and the bacteria is "clinging" to those streaks.

    Nope, still there
    [​IMG]Vibrant_part2-0027 by Jason, on Flickr

    [​IMG]Vibrant_part2-0029 by Jason, on Flickr
     
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  6. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    So, now for some stain. Just did a basic gram stain process: cleaned the slide, added the sample, burned it onto the slide, added some violet, rodi rinsed, added some iodine, rodi rinsed, light ethyl alcohol rinse, rodi rinse, add some safranin, final rodi rinse, and dry.

    And, after all that, still there. But in this third sample for the night I found a monster of a strand.

    [​IMG]Vibrant_part3-0051 by Jason, on Flickr

    [​IMG]Vibrant_part3-0052 by Jason, on Flickr

    [​IMG]Vibrant_part3-0053 by Jason, on Flickr

    [​IMG]Vibrant_part3-0054 by Jason, on Flickr

    [​IMG]Vibrant_part3-0056 by Jason, on Flickr

    [​IMG]Vibrant_part3-0057 by Jason, on Flickr

    [​IMG]Vibrant_part3-0060 by Jason, on Flickr

    [​IMG]Vibrant_part3-0061 by Jason, on Flickr

    [​IMG]Vibrant_part3-0065 by Jason, on Flickr

    [​IMG]Vibrant_part3-0066 by Jason, on Flickr
     
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  7. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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  8. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    And last night I took a look at the 3rd sample I've been wanting to get to and found something that now makes me wonder if the strands above in the Vibrant bottle aren't algae based. I wasn't think it was algae based at all as they are thin to extremely thing strands.

    BUT I found this (more photo's from last night to come)

    Some kind of strand seen in the algae
    [​IMG]Hair_Algae_Pump-0070 by Jason, on Flickr

    Chains of bacteria coming off it
    [​IMG]Hair_Algae_Pump-0071 by Jason, on Flickr
     
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  9. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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  10. UWC

    UWC Well-Known Member R2R Supporter Gold Sponsor

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    If they were more uniformed shaped, we'd call them wipers :p :D
     
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  11. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    The purple does confirm what was already suspected of the gram positive nature of streptococcus.

    [​IMG]Vibrant_part3-0056 by Jason, on Flickr
     
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  12. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Can you confirm that those strands are not something I'm doing to contaminate the samples? Or even confirm what those strands are that bacteria are clinging to? Email is fine too? ;)
     
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  13. UWC

    UWC Well-Known Member R2R Supporter Gold Sponsor

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    You are doing just fine :cool:
     
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  14. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Are you refering to something like a microfiber cleaning wiper?
     
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  15. UWC

    UWC Well-Known Member R2R Supporter Gold Sponsor

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    Windshield wipers :)
     
  16. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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  17. Elementalj

    Elementalj Well-Known Member R2R Supporter

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  18. UWC

    UWC Well-Known Member R2R Supporter Gold Sponsor

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  19. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Totally understood.
     
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  20. Rick.45cal

    Rick.45cal Well-Known Member R2R Supporter R2R Excellence Award

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    Pretty clever UWC
     
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