Our tank's biology up close

https://www.triton.de/en/

jason2459

Not a paid scientist
View Badges
Joined
Jul 28, 2015
Messages
4,262
Reaction score
2,706
A few weeks ago my microscope broke. It wasn't as old as PaulB's relic (I think he calls it an antique) but it was several decades old. So, I was then overwhelmed and excited with all the new options out there today and got a trinocular microscope with a 5MP camera. 40-2500x. Much stronger then I was using before.

I love looking at this stuff and now I can easily take pictures and videos of it all. Figured I'd share and maybe someone else might enjoy. I'll post stuff up periodically. Since there's no biology section I figure the Chemistry section will have to do... ha!

Cyano and some of the creatures living in it.

Sample was maybe around 1mm

~40x

Can see the blurry swirls of the bigger creature I got images of in the next post.






 
Last edited:
Top Shelf Aquatics
OP
jason2459

jason2459

Not a paid scientist
View Badges
Joined
Jul 28, 2015
Messages
4,262
Reaction score
2,706
~1000x (at 2500x I could see a ton of micro organisms but the camera is limited to 1000x right now with my setup but a lot more life can be seen)


In the thick of it. Above was on the edges. This looked like snakes intertwining







Few critters here



This would twist like a corkscrew to move




There was a lot of these swimming around





See if you can find it in the next two pictures. This very thin filament shoots across almost like its charged with electricity and corkscrewing at the same time. Very excited critter.



 
OP
jason2459

jason2459

Not a paid scientist
View Badges
Joined
Jul 28, 2015
Messages
4,262
Reaction score
2,706
Then also saw a lot single celled organisms and protists with flagellates I don't think the camera was able to pick up as I saw it with the 25x WF lens in at 2500x. Spent many hours exploring that tiny sample of cyano last night. I'll see what I can do for the video taken.
 
OP
jason2459

jason2459

Not a paid scientist
View Badges
Joined
Jul 28, 2015
Messages
4,262
Reaction score
2,706
Thanks and thanks.

Did you use any color dye?

No I didn't for this sample. I was able to see more then enough life moving around I was so excited to get so up close with and forgot all about it at the end. I totally didn't eat dinner till like 2am. I think I'm going to sample some white bacteria I have plenty of in my sump from carbon dosing next. I'll probably use Methylene blue for that.


Edit: I did manipulate the diaphragm and condenser to improve contrast here and there though.


This is the microscope and kit I got. My kids had fun with the kit and prepared slides.

http://www.amazon.com/gp/product/B01BPJJ70I?psc=1&redirect=true&ref_=oh_aui_detailpage_o03_s00
 
BRS
OP
jason2459

jason2459

Not a paid scientist
View Badges
Joined
Jul 28, 2015
Messages
4,262
Reaction score
2,706
Thanks for sharing. Great pictures. I've been thinking of getting a digital microscope and this sealed the deal for me :)

Thanks. It's great having that digital camera. I can capture and look back now and the kids love it as they can see on my laptop display. Though my son still likes looking in the eye pieces.
 

brandon429

why did you put a reef in that
View Badges
Joined
Dec 9, 2014
Messages
17,497
Reaction score
14,206
Location
tejas
excellence
the cyano pics
there are cell walls in that pic and organelle packets and chlorophyll et al?

that's not to say it didn't come from a patch of cyano, but wow that looks like algae and communities of more than one invader in a patch are routine, just musing. I was expecting oil immersion microscopy to be required to see the monera but that could be wrong, all I have is an antique one too at 100x max.
 
Last edited:
Lazys Coral House
OP
jason2459

jason2459

Not a paid scientist
View Badges
Joined
Jul 28, 2015
Messages
4,262
Reaction score
2,706
excellence
the cyano pics
there are cell walls in that pic and organelle packets and chlorophyll et al?

that's not to say it didn't come from a patch of cyano, but wow that looks like algae and communities of more than one invader in a patch are routine, just musing. I was expecting oil immersion microscopy to be required to see the monera but that could be wrong, all I have is an antique one too at 100x max.

Could be. But also to note those pictures in post 2 and 3 were fairly dried out at that time and cells were breaking apart. Otherwise those larger long creatures were way to fast to get a clear picture like that. Video I still need to edit and compress to upload shows them moving all around in the cyano and possibly, just my guess, eating it.

The 1000x is with oil immersion. The camera setup seems to me very similar to having the 10x WF eye pieces in.

There was definitely a lot of life in that 1 little millimeter.

Edit: in the first picture you can see one of those almost right in the middle. I's a blur that curves around on top. It curves around in and out of it a lot.

Incredible!! Please keep this up!

Thanks! I will be working on the video first and then taking some more samples and will be sure to share them in this thread. I was thinking the white bacteria strands in my sump but also very curious about sampling some skimmate that's very thick in my collection cup as I haven't cleaned it out in many many months as it drains to a 5g bucket and have a neck cleaner which is another "experiment" I'm doing called laziness.
 
Last edited:
OP
jason2459

jason2459

Not a paid scientist
View Badges
Joined
Jul 28, 2015
Messages
4,262
Reaction score
2,706
Here's the pic highlighting





Now this thing was funny. It would switch directions all the time zipping all around spiraling very fast to move. It seemed stiff but flexible if that makes sense like lightning. It seemed very energetic like it was charged with electricity.


 
OP
jason2459

jason2459

Not a paid scientist
View Badges
Joined
Jul 28, 2015
Messages
4,262
Reaction score
2,706
Uploading first video that's really not good at all. I haven't had a chance to look at all the videos yet but this is the only one that got copied over to a drive I can get to to edit the video. This was originally over 5 minutes long and a LOT more movement and blurring. I'll have to keep in mind the 16fps of the video at the higher resolution next time. I was just concentrating at exploring the sample and taking a few pictures. Hopefully the other videos are a bit better and I'll try better next time.

It's not fully uploaded yet but says this is the url for it. So should be live in the next 20 minutes or so.
 
https://www.triton.de/en/

Do you use any type of LIQUID additives, supplements, foods, medicine etc. for you reef aquarium?

  • Yes LIQUID

    Votes: 363 88.5%
  • NO

    Votes: 37 9.0%
  • Yes but not a LIQUID

    Votes: 7 1.7%
  • Other (please explain)

    Votes: 3 0.7%

Online statistics

Members online
2,008
Guests online
5,082
Total visitors
7,090
CK
Top