Our tank's biology up close

Discussion in 'Reef Chemistry by Randy Holmes-Farley' started by jason2459, Mar 15, 2016.

  1. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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  2. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Going through layers of my sandbed now. I sucked up a sample from about 3/4" to 1" down at around the thickest area of my sandbed which also happens to be along the front of the glass.

    I found a few things I had not seen before from my tank . That's always exciting. One if which showed me the origin of something I had seen previously. I will have to go back to some previous videos and watch again.


    I got something over 200 pictures and many videos. Quite a few on the last thing I found just as I was about to shut down for the night.
    [​IMG]
     
  3. Husker

    Husker Well-Known Member R2R Supporter

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    Love this thread. We just got an SEM, so I'm planning on taking in some reef related pieces to test it all out once we get it up and running.
     
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  4. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    I'll try not to post all 209 pictures... lol I usually skim those that are terrible which many often are. flickr has the full album

    and click on links for full resolution

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0001 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0003 by Jason, on Flickr

    A rotifer I believe. First I've seen one on this kind under a microscope. But it's also the first time I've starting going into my sandbed I think.
    [​IMG]Layers_of_a_Sandbed_1inch_Down-0004 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0008 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0012 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0015 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0018 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0020 by Jason, on Flickr
     
  5. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Lots of diatoms. I like diatoms as they are not a pest in my tank. I haven't had a diatom bloom in ages. They are an important link in the system. IMO

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0021 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0023 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0024 by Jason, on Flickr

    some detritus for life to live off of is not a bad thing either.
    [​IMG]Layers_of_a_Sandbed_1inch_Down-0026 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0027 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0030 by Jason, on Flickr


    I would wager every aquarium and body of water on this plant has some level of cyano. Even the white patch of sane I pulled this sample from.
    [​IMG]Layers_of_a_Sandbed_1inch_Down-0036 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0037 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0040 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0041 by Jason, on Flickr
     
  6. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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  7. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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  8. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Another critter I have not seen from my tank before! This sucker was FAST. I man it would zoom around at a pretty good speed and then instantly jump across at amazing speeds for something that looks so clunky. Again I think it's another type of Rotifer? It was hard getting video but I think I have some. I either ran into the same one twice or found two with the second being towards the end of my session.

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0068 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0069 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0070 by Jason, on Flickr
     
  9. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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  10. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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  11. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    I was at 100x when I ran into this pod. And decided to try and follow it. It wasn't easy at times but hopefully in the video it captured well what I was seeing. Which was getting to see it eat and swallow some food(detritus) and the guts moving things along.

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0082 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0085 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0087 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0092 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0094 by Jason, on Flickr

    Then switched to 60x making it a little easier to follow around but still mighty close up.

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0101 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0102 by Jason, on Flickr


    Piece of detritus just entered the mouth here.
    [​IMG]Layers_of_a_Sandbed_1inch_Down-0104 by Jason, on Flickr
     
  12. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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  13. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Then I start to just play around with the darkfield. And plaing around with the gain and exposure in the software. I found the lower the gain the darker the dark but harder to see more stuff. The higher the gain I got to see a wonderful amount of bacteria but all the other stuff over exposed even with the exposure turned down. But it was, as mentioned by someone else, like looking at the night sky.

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0137 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0140 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0143 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0144 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0145 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0148 by Jason, on Flickr
     
  14. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    And then I landed on the creature that freaked me out when I saw it in the darkfield. All of a sudden I see the washed out over exposed blotch with "tentacles" reaching out all over and it was slowly moving around. And I proceeded to follow this thing for a very long time.

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0153 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0154 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0155 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0156 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0157 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0159 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0160 by Jason, on Flickr
     
  15. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Went back to brightfield to see what the heck this thing was. Still don't know. But the "Feelers" were so interesting to watch as it seemed almost like a nerve or something and would pass up and down information it seemed. And they could morph and branch out. It was crazy. I hope the video's show it all well. I bet I have some long ones.

    But these feelers or what ever they are I HAVE seen before. And will have to go back and review the videos again to see if I can spot this thing that it seems is the origin. Or more then one organism in my tank can do the same thing.

    Got what looks like a lot of blurry shots but I was trying to focus on the odd extensions. I was pushing the contrast optically as much as possible. Both Irises nearly fully closed and exposure turned up.
    [​IMG]Layers_of_a_Sandbed_1inch_Down-0162 by Jason, on Flickrv


    [​IMG]Layers_of_a_Sandbed_1inch_Down-0164 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0165 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0168 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0169 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0171 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0173 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0177 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0178 by Jason, on Flickr
     
  16. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    The direction the thing headed always seemed to have it's longest "feeler" out ahead

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0181 by Jason, on Flickr

    Kept trying to see an entry point and almost seems like there's channels built into it.
    [​IMG]Layers_of_a_Sandbed_1inch_Down-0184 by Jason, on Flickr


    It was careful to navigate around things or stop when something like a diatom was heading towards it. But the extensions never touched them.

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0186 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0187 by Jason, on Flickr
     
  17. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Then the thing start flipping around and I was freaking out trying to get more details.
    [​IMG]Layers_of_a_Sandbed_1inch_Down-0188 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0190 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0191 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0192 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0193 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0200 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0203 by Jason, on Flickr

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0204 by Jason, on Flickr

    And then it just stayed in one spot of a long time. I kept hoping it would split or something. But never did. Even the feelers went away until one finally popped out and started just moving around again.

    [​IMG]Layers_of_a_Sandbed_1inch_Down-0208 by Jason, on Flickr
     
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  18. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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  19. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    What I believe is a type of rotifer ?








     
  20. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Several videos of diatoms and some cyano





     
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