Our tank's biology up close

Discussion in 'Reef Chemistry by Randy Holmes-Farley' started by jason2459, Mar 15, 2016.

  1. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Tried to get the pod in darkfield.

     

  2. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Couple perameciums chilling with some diatoms


    Not exactly sure what this was. Possibly the same thing I caught up with later that I think may be a rotifer as well. ? This one though is slightly different. Maybe just a basic ciliate?
     
  3. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Interesteing shape at 3:31. Maybe something to do with what I found at the end. Maybe not.

    Otherwise I caught back up with that mystery things in the last post closer up. It's very interesting looking. Almost crustacean like when looking at it from the side when it flips around.

    At 3:38

    Rotifer? Walking Ciliate? Some type of crustacean?



     
  4. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Not exactly sure what this is from and it seems like a molt from something? but I like the patterns and colors.

     
  5. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    So, here I am at 100x and I end up viewing the business end of a pod again... lol

    This time I was trying to focus more on it eating which was pretty cool




     
  6. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Another thing I had not seen before. Well, I've seen these before but not with flagella like things sticking out and moving. Possibly just seeing it for the first time with better optics. Now I'm curious what it is.

    First few seconds is the most exciting. After that it just sat there doing nothing like I'm used to seeing.
     
  7. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Found this critter twice and I think it's a type of rotifer. It can move very very fast it seems. First video is the first one I found and didn't jump around like the second one I found.

    1st encounter



    2nd Maybe the same as the first one or a different one. Very hard to keep focused on and follow


    Zoomed out and it would still just kind of twitch and bam it's in a new spot.
     
  8. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Then started playing around with the darkfield again and playing with the gain in the software. Made lots of things over exposed but brought out other things well.



    This was cool. Not exactly sure but it seemed like the cyano was trailing off new strains behind it.




    Could really see some of the bacteria and smaller organisms moving around. Like exploring through space.
     
  9. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    While I was playing around with the darkfield I ran accross the weird overexposed object except it had feelers, for a lack of a better word, stretching out all around it. I went back to darkfield again toward the end of this video. I didn't have it recording when I first ran into in darkfield.











     
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  10. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Man getting all these uploaded has been challenging. First a unplanned reboot killed my uploads, then ran out of battery thinking laptop was plugged in killed the uploads again, then a forced windows update and reboot killed the uploads again, and then was on spotty wifi and kept stalling the uploads...

    2 of the samples have been uploaded finally. Still getting the rest of what I captured uploaded...and it's the mystery black mat.


    So, here's what I've gotten uploaded so far. Samples from member karimwassef.

    [​IMG]

    [​IMG]

    Each sample I sliced off a chunk and sucked up some with my pipette as usually.

    Have wide cover slips just for this.
    [​IMG]
     
  11. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Full Sample 3 album here
    https://www.flickr.com/photos/145000273@N07/albums/72157685924183971

    and click on the links with the pictures to get full resolution.

    [​IMG]Coral_Tip_Sample03-0001 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03-0002 by Jason, on Flickr

    Looks like some kind of brown algae with the green algae.
    [​IMG]Coral_Tip_Sample03-0003 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03-0003 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03-0004 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03-0005 by Jason, on Flickr

    Typical diatoms
    [​IMG]Coral_Tip_Sample03-0006 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03-0007 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03-0008 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03-0009 by Jason, on Flickr
     
  12. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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  13. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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  14. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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  15. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    [​IMG]Coral_Tip_Sample03-0040 by Jason, on Flickr

    This is what I believe to be a type of cyano but haven't seen it in my system. A lot more of it in the other samples.
    [​IMG]Coral_Tip_Sample03-0041 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03-0042 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03-0043 by Jason, on Flickr

    Sometimes the pictures look out of focus but if you look around you'll see what I was focusing on. :)
    In this case it was the spirochete bacteria. They are pretty cool.
    [​IMG]Coral_Tip_Sample03-0044 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03-0045 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03-0046 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03-0047 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03-0048 by Jason, on Flickr
     
  16. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Here's the thick mass taken from the bag. Lots of cyano mixed with algae

    [​IMG]Coral_Tip_Sample03-0049 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03-0050 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03-0051 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03-0052 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03-0053 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03-0054 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03-0055 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03-0056 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03-0057 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03-0058 by Jason, on Flickr
     
  17. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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  18. Brew12

    Brew12 Electrical Gru R2R Supporter Reef Squad Leader R2R Excellence Award Build Thread Contributor Article Contributor Partner Member 2018

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    Great work as always, Jason!

    I have to ask. What magnifications do you find most useful for algae and bacteria identification?
     
  19. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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  20. jason2459

    jason2459 Not a paid scientist R2R Supporter R2R Excellence Award

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    Video on flikr for now and will get up to youtube later. Click on the picture or link to see the video.

    [​IMG]Coral_Tip_Sample03_01 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03_02 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03_03 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03_04 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03_05 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03_06 by Jason, on Flickr

    [​IMG]Coral_Tip_Sample03_07 by Jason, on Flickr


    There is one more video but looks like it failed to upload. I'll work on that once the black mat pictures and videos are up.
     
    Last edited: Sep 14, 2017
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