One more dumb question, if you put the container right on the white paper, it appears darker then when you hold it slightly above the White paper. in good light, holding the container just above the white paper seems to mirror API results. I know API is considered substandard, but it’s nice when things are consistent
In my opinion - the difference doesn't matter as long as you do it the same way every time. The relative trend over time matters.
API is fine for nitrate as well, because the nitrate level only matters in extremes. If you have zero, that's bad, if you have several hundred, that's also bad. If you have like 5-50, it's fine.
The difference between 20 or 25 doesn't matter in the slightest for nitrate.