Aquaforest Bio S PCR Electropherogram

[mcgullen]

I am submitting additional samples of bottled nitrifying bacteria for 16s sequencing and noticed that Aquaforest Bio S (Sample 3) has no reaction on PCR Electropherogram. Is my bottle dead or is the product never meant to contain live strains? The latter would be weird as the box clearly states "concentrated nitrifying bacteria." Does anyone have information on this? Thanks.

 

[taricha]

some products clump and take a lot of shaking to get them evenly distributed.
After you shake and pour out a sample, does it centrifuge to a visible pellet?
I'm wondering if the amount of actual live bacteria is very very small would it show up? ( I think the answer is yes, PCR multiplies whatever is there - but I don't have experience with those technologies.)

Also, if it were a different kind of bacteria than what you are hunting, would it show up on that chart?
"Bio S contains specially selected bacterial strains that create a perfect environment for replication of Nitrospirae and Nitrobacteraceae bacteria which are responsible for the nitrogen cycle."

"creates an environment for..." sounds like it is not a source of those nitrifying bacteria, but is meant to assist them in some unspecified way.