Here are the AVMA euthanasia guidelines (p. 83).
Jay
Jay
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"(5) Maceration (1 step). When applied correctly, using a well-maintained macerator specifically designed for the size of fish being euthanized, death is nearly instantaneous.324 The process is aesthetically unpleasant for some operators and observers."
Manually applied blunt force trauma (cranial concussion; Figure 24) followed by pithing or exsanguination (2 step). Manually applied blunt force trauma (a rapid, accurately placed blow of sufficient energy to the cranium with an appropriate-sized club) can cause immediate unconsciousness and potentially death, but should be followed by pithing or exsanguination to ensure death. The fish’s size, species, and anatomy and characteristics of the blow (including its accuracy, speed, and club mass) will determine the efficacy of manually applied blunt force trauma. This procedure requires training and monitoring for proficiency. Anatomic features, such as the location of the eyes, can help serve as a guide to the location of the brain.322,323
I'm surprised this wasn't removed along with the others. That isn't a humane way of euthanasia and shouldnt really be recommended. It takes time and between the time of co2 entering the water and death the fish is suffocating and in a lot of stress.Club soda!
Put the sick fish in a small container with aquarium water. Plastic bag is probably best?
Shake the bottle, put the screw top under water, open!
The carbonation will fill the water column with CO2.
Fish will black out very quickly. Death in a few minutes unless you allow it to revive in oxygenated water.