Manually applied blunt force trauma (cranial concussion; Figure 24) followed by pithing or exsanguination (2 step). Manually applied blunt force trauma (a rapid, accurately placed blow of sufficient energy to the cranium with an appropriate-sized club) can cause immediate unconsciousness and potentially death, but should be followed by pithing or exsanguination to ensure death. The fish’s size, species, and anatomy and characteristics of the blow (including its accuracy, speed, and club mass) will determine the efficacy of manually applied blunt force trauma. This procedure requires training and monitoring for proficiency. Anatomic features, such as the location of the eyes, can help serve as a guide to the location of the brain.322,323