Hi, so I was setting up my new 60-litre saltwater tank. It has dry white sand, and 1 dry sansibar rock. I had begun fishless cycling two weeks back ( with lab grade 25% ammonia solution). Since the water volume is 45 litres, I calculated I needed a little less than 0.5 ml of the ammonia solution to get a 2 ppm concentration.
The issue was that I was using a Salifert kit, and apparently it gives the free ammonia concentration ( though it claims to measure TAN) [ as a lfs, some internet forums said]. ( I got to know this much later) Since the salifert kit did not show a proper 2 ppm reading, I redosed the tank with another 0.3 ml of the ammonia solution.
On top of that, I had added 2 algae wafers + 5-10 pellets in order to ensure other nutrients are available for the growth of the bacteria. I had added a local brand of bacteria in a bottle ( containing Nitrobacter, Nitrosomonas)
My readings:
Since I was expecting the rate of nitrite drop to speed up..but nitrite remained unchanged after a day, although Ammonia has dropped to 0, I am worried. It appears as if the NOBs have not established themselves as well as the AOBs.
Now, I am worried that my AOBs will starve and my tank will get “uncycled”….. Also, I am worried if I redose more ammonia, my nitrates will be sky high, and I have to throw away a huge amount of saltwater ( which might hurt my bacteria population + budget). I was hoping the nitrite would drop to 0 soon, and I will do a stress test and then add livestock right away ( after doing a 50% water change)
I have a huge ton of diatoms in the top filter floss ( I let them be..so that they can absorb some of the nitrates..I plan to add chaeto in the top filter box ( as it has good flow + light)). The salinity is approximately 32 ppt, I can’t tell the current pH ( my meter broke)..but it was initially 8.3 approx
Could anyone suggest the next course of action that I should take? Is anything interfering with my cycle? I read on ChatGPT that high nitrate can stall the cycle ( but I can’t vouch for the authenticity of the statement, as I found no other backing)
The issue was that I was using a Salifert kit, and apparently it gives the free ammonia concentration ( though it claims to measure TAN) [ as a lfs, some internet forums said]. ( I got to know this much later) Since the salifert kit did not show a proper 2 ppm reading, I redosed the tank with another 0.3 ml of the ammonia solution.
On top of that, I had added 2 algae wafers + 5-10 pellets in order to ensure other nutrients are available for the growth of the bacteria. I had added a local brand of bacteria in a bottle ( containing Nitrobacter, Nitrosomonas)
My readings:
| Days | Salifert Ammonia | Salifert No2- | Salifert No3- | API Ammonia |
| 1 ( after dosing) | 0.25 | 0.025 | 2.5 | - |
| 3 | 0-0.15 | 0.05 | 5 | - |
| 5 | 0-0.15 | 0.1 | 5 | - |
| 7 | - | 0.5 | 10 | More than 4 ppm , less than 8 ppm |
| 10 | - | 2 | 25 | 4 |
| 13 | - | 1 | 50 | 0 |
| 14 | - | 1 | 50 | 0 |
Since I was expecting the rate of nitrite drop to speed up..but nitrite remained unchanged after a day, although Ammonia has dropped to 0, I am worried. It appears as if the NOBs have not established themselves as well as the AOBs.
Now, I am worried that my AOBs will starve and my tank will get “uncycled”….. Also, I am worried if I redose more ammonia, my nitrates will be sky high, and I have to throw away a huge amount of saltwater ( which might hurt my bacteria population + budget). I was hoping the nitrite would drop to 0 soon, and I will do a stress test and then add livestock right away ( after doing a 50% water change)
I have a huge ton of diatoms in the top filter floss ( I let them be..so that they can absorb some of the nitrates..I plan to add chaeto in the top filter box ( as it has good flow + light)). The salinity is approximately 32 ppt, I can’t tell the current pH ( my meter broke)..but it was initially 8.3 approx
Could anyone suggest the next course of action that I should take? Is anything interfering with my cycle? I read on ChatGPT that high nitrate can stall the cycle ( but I can’t vouch for the authenticity of the statement, as I found no other backing)

