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The idea stands or falls on the dilution rate in the refugia. What is it, approximately, amongst friends?It’s just a hypothesis, but I dosed upstream of my refugia and never saw any bacteria in the display. I’m speculating it grew on the rocks in my refugia. It seems logical that the bacteria will thrive most where there is the most organic, before it dilutes into the whole tank system.
The second aspect I struggle to think quantitatively about is the distribution of consumption. It likely takes hours to consume a 1 mL vinegar dose in one gallon. I assume you are doing a continuous dose. I think this means a very dilute organic carbon solution incompletely washes over refugia surfaces as it is diluted. I guess the planktonic bacteria are more likely consuming it because they live with this organic soup and are not seeing it briefly from only one side of a biofilm. Then there is the diffusion rate issue and poor circulation at the surfaces with a thick stagnant layer which further slows consumption by the biofilm.
The lack of visible bacteria growth is likely from not doing bolus doses-though if you are doing bolus doses, I need to come up with another reason :-) When dosing a model system, it is only during exponential growth that bacteria becomes visible. If your system is not at exponential growth, I doubt you will see bacteria growth no matter how you dose.
