Phytoplankton keeps dying off

[LiveFreeOrReef]

I've tried a couple of times to start up a phytoplankton culture using a jar of live phytoplankton from algae Barn on one attempt and dinkins on another attempt. Both times my phytoculture is crashed. I'm confused on what I'm doing wrong? I use the Coral ranch f2 fertilizer and I only use two MLs for the 2,000 ml container I'm using. I'm mixing the saltwater properly. I'm not using a heater and I'm using an air stone running very slowly. I also stir the contents everyday. I have attached pictures of what it looked like after I started the culture and what it looks like now.

 

[Fish Fan]

Hello!

Do you sanitize your vessel and other equipment?

Check out @EnterName's recent phyto culture guide for additonal help:
https://www.reef2reef.com/threads/another-phytoplankton-culture-tutorial.1165682/

Good luck!

 

[JTP424]

Couple of questions!!!
Are you using a single strain or mixed?
Do you have a light source for it?
*Edit, also do you keep the lid cockeyed like that?

 

[Mr. Mojo Rising]

Just asking cause its not mentioned in your post, but do you have a light on it?

 

[JTP424]

ahhh looks like there are little lights underneath... not sure if they're grow lights with the proper spectrum....
Based on thoughts...

If its from Algaebarn it's potentially oceanmagik, which is a blend, which the algaes will compete with each other, which IMO would just crash them all

If those aren't grow lights, the spectrum isn't proper for the algae to grow

If that's the air escape...(offset lid) you're probably getting contaminants killing off the algae

So potentially one to all three of those are my guess....