The concoction is comprised of
- Bacteria
- Particulate Food
- Carbon Source
- Trace Elements
- Zeozym
You can choose any product to fulfill those requirements. I have found some to be more useful than others, but the recipe does vary due to seasonality and what I have available on hand. The effects aren't too varied as long as you have at least the 5 components I listed. You may have different reactions depending on the dosage of each product, but that should be best left to individual to decide for their system. They should monitor their N and P to see that there is adequate nutrient cycling and that Nitrate does not remain elevated for prolonged periods in time.
I have not added in Calcium carbonate, but from my understanding it is nothing more than an inert powder that serves as way to transport bacteria. It is used to dust surfaces. The zeozym and some of the particulates do not completely dissolve so I feel that there is less of an incentive to use Calcium carbonate. However, if you do decide to add it in, let me know how it goes.
Zeozym acts like an enzyme, I don't think its an antibiotic and it definitely does expedite the processing of decaying matter. If you make the concoction, put in the zeozym last. When you finally put in the zeozym is when you'll really notice a chemical reaction take place. Due to this, it will also bring down the PH in the mixing vessel. With the amount of zeozym I use in my mix, the concoction has a PH of around 5.
When dosing into the tank I have sprayed it directly over corals, but to ere on the side of caution I would avoid direct contact, and to broadcast feed in front of a powerhead.
I've fed the concoction during all parts of the day, no need to limit yourself to a specific time. Eventually you can train your corals to feed at any point of the day. However, when you're first starting to use the concoction I would recommend you to dose at night. That way the corals are optimized for prey capture.
No PNS Pro Bio yet, but definitely interested in the product!