Trident test fails

[GillMeister]

Yeah, I know there are other threads on this.

I just hooked up my Trident now that I have room in my cabinet and I got the Test A Fail message. The sample line seems clear and my discharge collection container is getting material in it so I think it's all plumbed right. I restarted it. I primed it. Is there anything else I need to try?

 

[Yates273]

Did you use this to trouble shoot
https://www.neptunesystems.com/getstarted/trident/troubleshooting/

 

[Gtinnel]

Make sure the lines going to the reagent bottle isn’t pinched. It’s easy to do accidentally

 

[GillMeister]

I followed the troubleshooting instructions and I checked for pinched lines. I didn't use needle guides but those arrived in the mail yesterday. Could that be a factor?

 

[chimbo84]

Unlikely. Try taking the reagents out and make sure all the tubes are connected at the back of the unit. I had a tube detach at the back of the unit causing this exact issue.
The issue I am describing is similar to this:
https://forum.neptunesystems.com/showthread.php?27145-FIXED-No-Water-Sample-Err-Loose-tubing-inside

 

[GillMeister]

Unlikely. Try taking the reagents out and make sure all the tubes are connected at the back of the unit. I had a tube detach at the back of the unit causing this exact issue.
The issue I am describing is similar to this:
https://forum.neptunesystems.com/showthread.php?27145-FIXED-No-Water-Sample-Err-Loose-tubing-inside

I had checked this as well. Everything looked fine on the spider at the back of the unit.