Dinoflagellates - dinos a possible cure!? Follow along and see!

[StrangeDejavu]

Thats awesome and that was strained water?
Too cool!

Yup, I even tried it through a coffee filter but they went right through since the species I had (Amphidinium) were only 10 micron. They're a lot smaller than Ostreopsis and a few other species.

 

[twilliard]

Yup, I even tried it through a coffee filter but they went right through since the species I had (Amphidinium) were only 10 micron. They're a lot smaller than Ostreopsis and a few other species.

May I as.. when a person wants to know the size of a cell is is that dependant on the scope used or is there an attachment a person can get for a scope?

 

[StrangeDejavu]

May I as.. when a person wants to know the size of a cell is is that dependant on the scope used or is there an attachment a person can get for a scope?

I wouldn't be surprised if there was an eyepiece with a measurement reticle like that, but I determined off papers and a photo found on Google. It's probably more accurate to say 12 - 15 micron, but 10 is close enough. This is an image someone else took and I attached photos I took of my species to confirm ID.

 

[twilliard]

Thank you!
I am seeing that the software will do it for me just have to learn how to use it lol
Here is the new scope I am looking at
http://www.amscope.com/compound-mic...lar-compound-microscope-1920x1080-camera.html
I need a better illumination system

 

[reeferfoxx]

I might have dinos.

If you haven't taken the time to read through the thread, this treatment is in experimental phase. Twilliard has found a non abrasive method to eradication. He is attacking it's reproduction and eliminating reproduction. It isn't an overnight cure that will kill dinos. To the best of my knowledge, killing dinos can release toxins that can hurt the biology of your tank. He has identified his strain of dinos and is having much success. What we have learned is there are 2200+ species of dinoflagellates. Other members that are participating, have other species. However, they are all classified as protozoans.

 

[domination2580]

If you haven't taken the time to read through the thread, this treatment is in experimental phase. Twilliard has found a non abrasive method to eradication. He is attacking it's reproduction and eliminating reproduction. It isn't an overnight cure that will kill dinos. To the best of my knowledge, killing dinos can release toxins that can hurt the biology of your tank. He has identified his strain of dinos and is having much success. What we have learned is there are 2200+ species of dinoflagellates. Other members that are participating, have other species. However, they are all classified as protozoans.

What im thinking is use metro to stop reproduction, and syphon them out during a water change. Maybe a 3 day blackout

 

[reeferfoxx]

3 day black out is up to you. It's not necessary. We are doing 250mg metro per gallon or 2 spoons per gallon of MetroPlex for 3 days. 4th day you should notice a difference.

 

[StrangeDejavu]

So today is Day 3 on my Dad's BioCube 29 using Metronidazole against Amphidinium carterae and a Symbiodinium lookalike. He is dosing 3 spoonfuls of MetroPlex (70% Metronidazole). Only 2 doses and he says he's already seen improvements. His water, when viewed from the side, used to be heavily yellowed/brown (something I noticed when I battled dinos as well). He says his water is much clearer though it still has a slight yellow tinge. Metro appears to be working as planned in keeping them broken up and suspended as his filter floss is catching significantly more than it ever has:

Clownfish, Yellow Watchman Goby and Skunk Cleaner Shrimp show no change in behavior, still eating well. Coral and Ball Anenome are unaffected. CUC doesn't appear to be affected. Collonista snails and Spirobid worms don't appear to be affected. Mini-brittle stars are also fine, and Copepods are active. Two things i've noticed: 1) Metro appears to have decimated the pineapple sponge population that used to live in his rear chambers, 2) Whether coincidence or not, i'm also no longer seeing red flatworms all over his glass.

 

[domination2580]

3 day black out is up to you. It's not necessary. We are doing 250mg metro per gallon or 2 spoons per gallon of MetroPlex for 3 days. 4th day you should notice a difference.

Currently is just stopping the reproduction though correct?

 

[reeferfoxx]

Currently is just stopping the reproduction though correct?

We've noticed under the scope that once metro is introduced, the dinos become lethargic and disoriented. They no longer care to be in groups at this point. It's at day 4 that you'll be able to take a turkey baster and let them blow around the tank. Your mechanical filtration will collect the dinos in the water column.

 

[reeferfoxx]

So today is Day 3 on my Dad's BioCube 29 using Metronidazole against Amphidinium carterae and a Symbiodinium lookalike. He is dosing 3 spoonfuls of MetroPlex (70% Metronidazole). Only 2 doses and he says he's already seen improvements. His water, when viewed from the side, used to be heavily yellowed/brown (something I noticed when I battled dinos as well). He says his water is much clearer though it still has a slight yellow tinge. Metro appears to be working as planned in keeping them broken up and suspended as his filter floss is catching significantly more than it ever has:

Clownfish, Yellow Watchman Goby and Skunk Cleaner Shrimp show no change in behavior, still eating well. Coral and Ball Anenome are unaffected. CUC doesn't appear to be affected. Collonista snails and Spirobid worms don't appear to be affected. Mini-brittle stars are also fine, and Copepods are active. Two things i've noticed: 1) Metro appears to have decimated the pineapple sponge population that used to live in his rear chambers, 2) Whether coincidence or not, i'm also no longer seeing red flatworms all over his glass.

Nice work!

 

[domination2580]

We've noticed under the scope that once metro is introduced, the dinos become lethargic and disoriented. They no longer care to be in groups at this point. It's at day 4 that you'll be able to take a turkey baster and let them blow around the tank. Your mechanical filtration will collect the dinos in the water column.

Amd this can be introduced into the dt without harm to coral correct?

 

[reeferfoxx]

@taricha ran across this little piece...

Also, ran across paper looking at MTZ toxicity on a few aquatic species.
Good news, they dosed massive amounts (30x the amount recommended here) and couldn't kill copepods.
Which means at least one important class of dino grazers is unaffected by the drug.

Amd this can be introduced into the dt without harm to coral correct?

So far nobody has experience any negative side effects.

 

[StrangeDejavu]

Amd this can be introduced into the dt without harm to coral correct?

The OP tested this method in a frag tank, if that makes you feel any better. His coral look great in the "after" shots.

 

[twilliard]

So today is Day 3 on my Dad's BioCube 29 using Metronidazole against Amphidinium carterae and a Symbiodinium lookalike. He is dosing 3 spoonfuls of MetroPlex (70% Metronidazole). Only 2 doses and he says he's already seen improvements. His water, when viewed from the side, used to be heavily yellowed/brown (something I noticed when I battled dinos as well). He says his water is much clearer though it still has a slight yellow tinge. Metro appears to be working as planned in keeping them broken up and suspended as his filter floss is catching significantly more than it ever has:

Clownfish, Yellow Watchman Goby and Skunk Cleaner Shrimp show no change in behavior, still eating well. Coral and Ball Anenome are unaffected. CUC doesn't appear to be affected. Collonista snails and Spirobid worms don't appear to be affected. Mini-brittle stars are also fine, and Copepods are active. Two things i've noticed: 1) Metro appears to have decimated the pineapple sponge population that used to live in his rear chambers, 2) Whether coincidence or not, i'm also no longer seeing red flatworms all over his glass.

That is spectacular!
Exactly how they responded in our tank.

 

[twilliard]

Thank you @reeferfoxx for keeping people updated I have been looking into a better scope. I got a sample in yesterday (bacteria) that my scope is having a hard time with.

 

[Greenstreet.1]

My last dose was Thursday have not seen any changes yet

 

[reeferfoxx]

Thank you @reeferfoxx for keeping people updated I have been looking into a better scope. I got a sample in yesterday (bacteria) that my scope is having a hard time with.

You're welcome! This is your niche. That sounds like a wise investment.

 

[reeferfoxx]

My last dose was Thursday have not seen any changes yet

Do you have any pics of your tank as of now?

 

[twilliard]

My last dose was Thursday have not seen any changes yet

Are you removing them now?
Drastic change in my tank didn't become noticeable till about day 10